Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2556697..2558285 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag K3072_RS12135 Protein ID WP_001125431.1
Coordinates 2556963..2558285 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag K3072_RS12130 Protein ID WP_001304368.1
Coordinates 2556697..2556963 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS12115 (2553065) 2553065..2554516 + 1452 WP_000854660.1 tagaturonate reductase -
K3072_RS12120 (2554713) 2554713..2555627 + 915 WP_001443133.1 bestrophin family protein -
K3072_RS12125 (2555631) 2555631..2556389 - 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
K3072_RS12130 (2556697) 2556697..2556963 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K3072_RS12135 (2556963) 2556963..2558285 + 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
K3072_RS12140 (2558595) 2558595..2559002 - 408 Protein_2390 acetylxylan esterase -
K3072_RS12145 (2559005) 2559005..2559586 - 582 Protein_2391 LysR family transcriptional regulator -
K3072_RS12150 (2559735) 2559735..2560517 + 783 WP_001221834.1 SDR family oxidoreductase -
K3072_RS25265 (2560761) 2560761..2560868 - 108 Protein_2393 hypothetical protein -
K3072_RS12155 (2561293) 2561293..2561856 + 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
K3072_RS12160 (2562218) 2562218..2562928 + 711 WP_001195181.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T212567 WP_001125431.1 NZ_CP081010:2556963-2558285 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T212567 NZ_CP107275:c5790547-5789678 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT212567 WP_001304368.1 NZ_CP081010:2556697-2556963 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT212567 NZ_CP107275:c5789685-5788852 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCACGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTCTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCACCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCAGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References