Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 4190094..4190773 | Replicon | chromosome |
| Accession | NZ_CP081009 | ||
| Organism | Escherichia coli strain Stbl4 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | K3830_RS20035 | Protein ID | WP_000854672.1 |
| Coordinates | 4190094..4190435 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | K3830_RS20040 | Protein ID | WP_000070395.1 |
| Coordinates | 4190456..4190773 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K3830_RS20010 (4185371) | 4185371..4185772 | + | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
| K3830_RS20015 (4185811) | 4185811..4186866 | - | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| K3830_RS20020 (4187154) | 4187154..4188257 | + | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| K3830_RS20025 (4188269) | 4188269..4189522 | + | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| K3830_RS20035 (4190094) | 4190094..4190435 | - | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| K3830_RS20040 (4190456) | 4190456..4190773 | - | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| K3830_RS20045 (4190792) | 4190792..4191013 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| K3830_RS20050 (4191022) | 4191022..4191498 | - | 477 | WP_000811693.1 | RadC family protein | - |
| K3830_RS20055 (4191514) | 4191514..4191972 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| K3830_RS20060 (4192070) | 4192070..4192309 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| K3830_RS20065 (4192386) | 4192386..4192853 | - | 468 | WP_001547765.1 | protein YkfB | - |
| K3830_RS20070 (4192876) | 4192876..4193319 | - | 444 | WP_000824223.1 | lipoprotein YafY | - |
| K3830_RS20075 (4193319) | 4193319..4193495 | - | 177 | WP_001285112.1 | hypothetical protein | - |
| K3830_RS20080 (4193542) | 4193542..4193733 | - | 192 | Protein_3957 | DeoR family transcriptional regulator | - |
| K3830_RS20085 (4193950) | 4193950..4194771 | - | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| K3830_RS20090 (4194863) | 4194863..4195726 | - | 864 | WP_001065553.1 | GTPase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4179843..4197676 | 17833 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T212542 WP_000854672.1 NZ_CP081009:c4190435-4190094 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T212542 NZ_CP107262:3516246-3516659 [Leclercia adecarboxylata]
GTGGTCCTGTGGCAATCTGATCTACGCGTCTCATGGCGCGCCCAGTGGATGTCGCTTCTGCTCCACGGCCTGGTCGCGGC
TGTTATTCTTCTGATGCCGTGGCCGCTGAGCTACACCCCATTATGGCTGATGCTGCTGTCGCTGGTGGTGTTTGATAGCG
TACGCAGTCAGCGGCGCATTAACGCCCGTCAGGGTGAAATCAAACTGTTTATGGATTCCCGTCTGCACTGGCAGGAGAGC
GAGTGGGAAATTGTCGGCACGCCGTGGATGCTGAACTCCGGCATGATGCTCCGCTTACGCAAAGGCGCGGGTCAACGCTG
CCATCATTTATGGCTTGCGGCAGACAGTATGGATGAAGCGGAGTGGCGGGATCTGCGTCGGATGATGTCGCACCAGCCGA
CGCAGGGGCGGTAG
GTGGTCCTGTGGCAATCTGATCTACGCGTCTCATGGCGCGCCCAGTGGATGTCGCTTCTGCTCCACGGCCTGGTCGCGGC
TGTTATTCTTCTGATGCCGTGGCCGCTGAGCTACACCCCATTATGGCTGATGCTGCTGTCGCTGGTGGTGTTTGATAGCG
TACGCAGTCAGCGGCGCATTAACGCCCGTCAGGGTGAAATCAAACTGTTTATGGATTCCCGTCTGCACTGGCAGGAGAGC
GAGTGGGAAATTGTCGGCACGCCGTGGATGCTGAACTCCGGCATGATGCTCCGCTTACGCAAAGGCGCGGGTCAACGCTG
CCATCATTTATGGCTTGCGGCAGACAGTATGGATGAAGCGGAGTGGCGGGATCTGCGTCGGATGATGTCGCACCAGCCGA
CGCAGGGGCGGTAG
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT212542 WP_000070395.1 NZ_CP081009:c4190773-4190456 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT212542 NZ_CP107262:3515999-3516265 [Leclercia adecarboxylata]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGTCGCGGCATGCGCGAACTCGATATCTCCATCATGCCGTT
CTTCGAATACGAATATGACAGCCTGAGCGATGAGGATAAGCATCTCTTCGTTCGCCTGCTGGAGAACGACGATCCCGATT
TATTTAACTGGTTGATGAATCACGGCAAACCCGCCGACGCCGAGCTGCAACGGATGGTGCTTTTAATTCAGACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGTCGCGGCATGCGCGAACTCGATATCTCCATCATGCCGTT
CTTCGAATACGAATATGACAGCCTGAGCGATGAGGATAAGCATCTCTTCGTTCGCCTGCTGGAGAACGACGATCCCGATT
TATTTAACTGGTTGATGAATCACGGCAAACCCGCCGACGCCGAGCTGCAACGGATGGTGCTTTTAATTCAGACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|