Detailed information of TA system TA021254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 3763423..3763985
• Replicon: chromosome
• Accession: NC_007413
• Organism: Anabaena variabilis ATCC 29413 (Anabaena flos-aquae UTEX 1444)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AVA_RS15365
• Protein ID: WP_011319780.1
• Coordinates: 3763644..3763985 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AVA_RS15360
• Protein ID: WP_011319779.1
• Coordinates: 3763423..3763647 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVA_RS15350	3760762..3761844	+	1083	WP_011319777.1	serine/threonine protein kinase	-
AVA_RS15355	3762009..3763373	+	1365	WP_011319778.1	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	-
AVA_RS15360	3763423..3763647	+	225	WP_011319779.1	DUF433 domain-containing protein	Antitoxin
AVA_RS15365	3763644..3763985	+	342	WP_011319780.1	DUF5615 family PIN-like protein	Toxin
AVA_RS15370	3764018..3764791	-	774	WP_011319781.1	hypothetical protein	-
AVA_RS15375	3765064..3765471	-	408	WP_011319782.1	hypothetical protein	-
AVA_RS15380	3765596..3766852	+	1257	WP_041456239.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (4-79)

Antitoxin

• Protein of unknown function DUF433 (8-63)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12430.35 Da
• Isoelectric Point: 4.2457

>T21254 WP_011319780.1 NC_007413:3763644-3763985 [Trichormus variabilis ATCC 29413]
MTTIWIDAHLSPVIATWINNTFGITALALRDIGLRDAEDLEIFEAAKAQGVIFITKDSDFVDLVERLGEPPKIIWLTCGN
TSNARLQEILSVTLPDALRLLQAGERLVEISGV

Nucleotide

• Length: 342 bp

>T21254 NC_007413:3763644-3763985 [Trichormus variabilis ATCC 29413]
ATGACAACGATTTGGATTGATGCACATCTGTCGCCGGTGATTGCTACCTGGATTAACAACACATTTGGGATTACAGCATT
GGCATTGCGTGACATTGGCTTGAGGGATGCTGAAGACCTGGAGATTTTTGAAGCAGCAAAAGCTCAAGGAGTTATCTTCA
TCACCAAAGACAGCGATTTTGTTGACTTAGTAGAACGCTTAGGAGAGCCACCAAAAATCATCTGGCTAACCTGCGGTAAT
ACATCAAATGCTCGTTTGCAAGAGATTCTGAGTGTTACACTACCGGACGCATTGAGGCTTCTACAAGCAGGCGAGAGATT
GGTAGAGATTAGCGGAGTTTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8104.53 Da
• Isoelectric Point: 7.8328

>AT21254 WP_011319779.1 NC_007413:3763423-3763647 [Trichormus variabilis ATCC 29413]
MSNLLQRITVNPKQCGGRPCIRGMRIRVSDVLDLLAAGLSSEQILAEMPDLEMDDLKASLAYAARKLNHPILTA

Nucleotide

• Length: 225 bp

>AT21254 NC_007413:3763423-3763647 [Trichormus variabilis ATCC 29413]
ATGTCAAACTTGTTGCAAAGAATCACAGTCAACCCTAAACAGTGTGGTGGTCGTCCCTGCATTCGTGGTATGAGAATTAG
GGTATCAGATGTACTTGATTTGTTAGCTGCGGGACTTAGCTCCGAGCAAATCTTAGCAGAAATGCCCGATTTAGAGATGG
ATGATTTGAAAGCATCACTCGCTTACGCTGCTCGTAAATTAAATCATCCCATCCTGACGGCATGA