Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3900818..3901649 Replicon chromosome
Accession NZ_CP081009
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K3830_RS18670 Protein ID WP_000854814.1
Coordinates 3901275..3901649 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag K3830_RS18665 Protein ID WP_001285584.1
Coordinates 3900818..3901186 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3830_RS18650 (3898485) 3898485..3900017 + 1533 WP_001350525.1 protein YeeR -
K3830_RS18655 (3900014) 3900014..3900460 + 447 WP_000187523.1 RadC family protein -
K3830_RS18660 (3900523) 3900523..3900744 + 222 WP_000692323.1 DUF987 domain-containing protein -
K3830_RS18665 (3900818) 3900818..3901186 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K3830_RS18670 (3901275) 3901275..3901649 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K3830_RS18675 (3901646) 3901646..3901840 + 195 WP_000988600.1 DUF5983 family protein -
K3830_RS18680 (3901886) 3901886..3901966 + 81 Protein_3677 hypothetical protein -
K3830_RS18685 (3902255) 3902255..3902383 - 129 Protein_3678 transposase domain-containing protein -
K3830_RS18690 (3902503) 3902503..3902637 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
K3830_RS18695 (3902738) 3902738..3903067 - 330 WP_000450409.1 DUF496 family protein -
K3830_RS18700 (3903239) 3903239..3904297 - 1059 WP_001200891.1 FUSC family protein -
K3830_RS18705 (3904495) 3904495..3904968 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
K3830_RS18710 (3905087) 3905087..3906253 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T212538 WP_000854814.1 NZ_CP081009:3901275-3901649 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T212538 NZ_CP107262:c2179407-2179123 [Leclercia adecarboxylata]
ATGACCTATGAGCTGGAATTCGATCCACGTGCGCTAAAGGAGTGGCAGAAACTGGGTGATACGGTTAAGAGTCAGTTTAA
GAAGAAGCTGGCAGTCGTCCTGTCAAACCCGCGAAGAGAGTCTGCCAGGCTACATGGCTTACCGGACTGCTACAAAATTA
AACTCAGTTCGTCCGGTTATCGGCTGGTATATCAGGTCAGGGATAAGGTCATTACGGTATTTGTGGTCGCAGTGGGAAAA
CGCGAAAAATCAGCCGTCTATCACGACGCAAATCAACGGCTTTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT212538 WP_001285584.1 NZ_CP081009:3900818-3901186 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT212538 NZ_CP107262:c2179639-2179397 [Leclercia adecarboxylata]
ATGGCCACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCATATGCCGTGCTGGAAAAACTGAACATTACCCC
GACGGAAGCCGTTCGGCTTCTGTTCCAGTATGTCGCGGAAAATGGCCGCATGCCGGTCAAAACCGTGACGGTAAGTGACA
GCGAAGATGAACTGATCAGGACGGTCAGGGAACGGCTGGCGAATCCGCAAAAAGGGATCAAGGTTAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References