Detailed information of TA system TA212537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3888140..3888665
• Replicon: chromosome
• Accession: NZ_CP081009
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: K3830_RS18610
• Protein ID: WP_001159868.1
• Coordinates: 3888360..3888665 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: K3830_RS18605
• Protein ID: WP_000813634.1
• Coordinates: 3888140..3888358 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3830_RS18590 (3883296)	3883296..3884195	+	900	WP_000963206.1	nucleotide-binding protein	-
K3830_RS18595 (3884245)	3884245..3886470	-	2226	WP_000698737.1	P-loop NTPase fold protein	-
K3830_RS18600 (3886472)	3886472..3887560	-	1089	WP_000952217.1	transcriptional repressor PifC	-
K3830_RS18605 (3888140)	3888140..3888358	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
K3830_RS18610 (3888360)	3888360..3888665	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
K3830_RS18615 (3888666)	3888666..3889472	+	807	WP_000016982.1	site-specific integrase	-
K3830_RS18620 (3890246)	3890246..3891001	+	756	WP_000852146.1	replication initiation protein RepE	-
K3830_RS18625 (3891589)	3891589..3892755	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3863022..3893726	30704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T212537 WP_001159868.1 NZ_CP081009:3888360-3888665 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T212537 NZ_CP107262:2038604-2039521 [Leclercia adecarboxylata]
ATGGATAGAACCTCCCCCTACCACCGCCAGGTCGCTTTGCTGGTGCGCGCACTACCCTATGTTGCCAACGAGTCCTGTTT
TGCGCTCAAAGGTGGAACTGCGATCAATCTGTTTATTCACAATTTTCCCCGATTGTCAGTTGATATCGATCTTGCCTACA
TACCCCTGGAAAGCAGGGAGGTGGCTCTGCCCAATGTACGCGATGCACTCACCCGCATCGCTAATGAACTGGAGGCTCAA
TCGGGGATCAGCGCAGTGCTGCAAACGAATAATCCCGACGAGATGCGCATCATTGTCACCACTGACGATGCACAAATTAA
AATTGAAGTTTCACCTGTCGCCCGGGGAACGTTGTATCCCCCGCAAGAACGGGATGTCGTGGAAACCGTAGAAGATGAAT
TTGGGTTTGCCGCGATCCCGGTCGTCTCACTCCCTGATTTGTATGGCGGCAAGTTATGCGCCGCGCTGGATCGTCAGCAT
CCCAGAGACTTTTACGATGTAAAAATGCTTCTTGATACACAAGGTATCGACCGCGCTATTTTCAATGGTTTTATCGCTTA
CCTGCTCAGCCATAATCGTCCTCTATCCGAGGTTCTTAGCCCGCGCTGGAAGGATATTACCGGGCCCTTTCAAAACGAAT
TTAACGGCATGACTATTCAGGATGTATCCCTTAAGGAGCTGGTTAACGTTCCGGAAAGAATGTTAAACGCGTTGAAAGAA
CATTTCACTCATCAGGATTTCGATTTTCTTATGTCCTTTAAACGTGGTGCTCCTGACTGGAATTTAGCGCCTGAAAGCCC
AATTCAACATTTGCCAGCCGTTCAATGGAAATTGCGCAATATCAGCAGGATGTCAGAAGAAAAACGCGCTCAGTCACTGA
AGAAACTGGAAAGGGTTCTGGAAAAGTGGATAAGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT212537 WP_000813634.1 NZ_CP081009:3888140-3888358 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT212537 NZ_CP107262:2037838-2038611 [Leclercia adecarboxylata]
ATGTCATCAAAATTAAACTGGCTACTGCAAAACACCTCTCCTGGCAGCCTGGTTTTGCAGTCCTGGCTGACACGAAACGA
CATCTCACCCTCTCTGGCATTCAAATATGCGCAAAATGGTTGGTTAAATAAACTGCGTGCCGGCGTTTATGCACGCGCGG
GGCGTGAACCGGACTGGAGCGATGCACTGGAATGTCTACAGAACCAGCTTGCTGCCCCTGTTTATCTGGCCGGACTAACC
AGCTTGTCCTGGCAAGGCCGTTCTCATTATCTACAGCTTAACCAGAGTCAATGCTGGCTTAGCGCTGAAAACAAAACGCT
GCTTCCAAAATGGTTCAAAGAATTTCCCGATGTTGACTGGCGAGTGGTATCAGGTCTGAAGCTTCCTCAACTGGACGAAA
AGTACCGTACTGAGCTTGAGATAAAAGGAAAAAAATTAACAGCCAGCGGGCCAGAACTAGCAACTTACGAACTGTTAAGC
GCAGTGCCTCAGGACATCTCATTTGAACACGCAGCCGAGCTTTTTCAGGGGCTGGTTAATCTTAACCCACGTAAAATAGA
GAGTTTGCTTGTGCTCAGCCAGGCAGTTCAAACCAAGCGTCTGTATCTATTTTTCGCCAGTTTCTATGAGCATGCTTGGC
TCAAACGCATTGATATCAGCAAAATTGACCTGGGATCGGGCAACCGACAAATCGTTGCCAATGGCAAGCTGAACACGCAA
TACCAGATCACCGTACCCGAAAATTTCAATCTGAAGGGAAGAAACTATGGATAG

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2