Detailed information of TA system TA212535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3696037..3696662
• Replicon: chromosome
• Accession: NZ_CP081009
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3830_RS17625
• Protein ID: WP_000911324.1
• Coordinates: 3696264..3696662 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: K3830_RS17620
• Protein ID: WP_000450532.1
• Coordinates: 3696037..3696264 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3830_RS17620 (3696037)	3696037..3696264	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K3830_RS17625 (3696264)	3696264..3696662	+	399	WP_000911324.1	type II toxin-antitoxin system VapC family toxin	Toxin
K3830_RS17630 (3696671)	3696671..3698824	-	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
K3830_RS17635 (3699077)	3699077..3699808	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
K3830_RS17640 (3699833)	3699833..3700354	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T212535 WP_000911324.1 NZ_CP081009:3696264-3696662 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T212535 NZ_CP107262:1383594-1383812 [Leclercia adecarboxylata]
ATGTCTGATAAACCATTAACCAAGACTGATTATTTGATGCGTTTACGACGCTGTCAGTCCATTGACACCCTGGAACGCGT
CATTGAAAAAAATAAATACGAGCTTTCTGATAATGAGCTTGCGGTATTCTATTCAGCAGCCGATCATCGTCTGGCTGAAC
TTACGATGAATAAGCTGTACGATAAAATCCCGACCGCTGTATGGAAGTTTGTACGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT212535 WP_000450532.1 NZ_CP081009:3696037-3696264 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT212535 NZ_CP107262:1383193-1383585 [Leclercia adecarboxylata]
ATGGACGAATACTCGCCTAAAAGGCAAGATATTGCACAGTTGAAATTTCTTTGTGAATCCCTGTACCATGACTGCCTGAC
CAATCTCGATGAAAGCAATCATGGCTGGGTAAATGACCCGACTTCTGCCATTAACTTGCAGTTAAACGAGTTGATTGAAC
ATATTGCTACCTTCGCGCTTAATTATAAAATTAAGTATAACGAAGATAATAAGTTGATTGAGCAAATTGACGAATACCTG
GATGACACTTTTATGTTATTCAGCAGCTACGGCATTAACGCACAGGATTTACAAAAATGGCGTAAATCGGGCAATCGGCT
TTTTCGTTGTTTTGTCAATGCAAGCAGAGCTAACCCGTTAGCTTCTCCGATTAAAATTATTACAAACAATTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5