Detailed information of TA system TA212530

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 2896854..2897351
• Replicon: chromosome
• Accession: NZ_CP081009
• Organism: Escherichia coli strain Stbl4

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: K3830_RS13745
• Protein ID: WP_001173343.1
• Coordinates: 2896854..2897027 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: L4JK92
• Locus tag: K3830_RS13750
• Protein ID: WP_000398619.1
• Coordinates: 2897055..2897351 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3830_RS13735 (2893524)	2893524..2894981	+	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-
K3830_RS13740 (2895218)	2895218..2896735	+	1518	WP_001295074.1	lysine--tRNA ligase	-
K3830_RS13745 (2896854)	2896854..2897027	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
K3830_RS13750 (2897055)	2897055..2897351	-	297	WP_000398619.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
K3830_RS13755 (2897578)	2897578..2897850	-	273	WP_000405651.1	GNAT family N-acetyltransferase	-
K3830_RS13760 (2897862)	2897862..2898092	-	231	WP_000371704.1	4Fe-4S mono-cluster protein YjdI	-
K3830_RS13765 (2898273)	2898273..2899904	+	1632	WP_001216456.1	sensor histidine kinase	-
K3830_RS13770 (2899901)	2899901..2900620	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T212530 WP_001173343.1 NZ_CP081009:c2897027-2896854 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T212530 NZ_CP107257:3033302-3034057 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAGGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT212530 WP_000398619.1 NZ_CP081009:c2897351-2897055 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT212530 NZ_CP107257:3032799-3033167 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTGGATGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41

Antitoxin

• AlphaFold DB: A0A7U9QBH6