Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1532829..1533483 Replicon chromosome
Accession NZ_CP081009
Organism Escherichia coli strain Stbl4

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K3830_RS07320 Protein ID WP_000244777.1
Coordinates 1533076..1533483 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K3830_RS07315 Protein ID WP_000354046.1
Coordinates 1532829..1533095 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3830_RS07290 (1527998) 1527998..1528741 + 744 WP_000951964.1 SDR family oxidoreductase -
K3830_RS07295 (1528798) 1528798..1530231 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
K3830_RS07300 (1530276) 1530276..1530587 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
K3830_RS07305 (1530751) 1530751..1531410 + 660 WP_000250274.1 hemolysin III family protein -
K3830_RS07310 (1531606) 1531606..1532586 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K3830_RS07315 (1532829) 1532829..1533095 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K3830_RS07320 (1533076) 1533076..1533483 + 408 WP_000244777.1 protein YgfX Toxin
K3830_RS07325 (1533523) 1533523..1534044 - 522 WP_001055874.1 flavodoxin FldB -
K3830_RS07330 (1534156) 1534156..1535052 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K3830_RS07335 (1535077) 1535077..1535787 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K3830_RS07340 (1535793) 1535793..1537526 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T212525 WP_000244777.1 NZ_CP081009:1533076-1533483 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T212525 NZ_CP107247:1869436-1869690 [Coxiella burnetii]
ATGAACGTAGTTACTTTTAGCGAACTCAGGGCGCAACTTAAAAAAATCTTGGATCTTTCCGCCGATCAACACGAACCTGT
CGTCGTTAAACGGCCAAATAAAGAAACCATGGTCATTTTATCTTTACGCGACTTTGAGGCTCTAAAAGAAACAGCTTATC
TCTTAAGTAACGAAGCTAATGCGGCCCGTCTTCGTCAGTCTATCCGCAGCTTAAAACAAGGCAAGGCACAAAAAAAGAAA
TTAATGGAAGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT212525 WP_000354046.1 NZ_CP081009:1532829-1533095 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT212525 NZ_CP107247:1869690-1869965 [Coxiella burnetii]
ATGCAAATTTCCTTCACGCCCGAAGCCTGGGAAGATTATTTATATTGGCAAAAATTCGACAAAAAAATGCTTCGACGCAT
TAATGAACTCATTAAGGATGCTATGCACGAGCCTTTTTCCGGAAAGGGAAAGCCAGAACCTTTAAAATTTGAATTACAAG
GATATTGGTCAAGACGATTAGATCAAGAACATCGATTGGTCTACAAAGTTTTAGACGATTCGTTAATGATTATCGCCGCA
AGATTTCACTATAATCGCCTTAATTCTAAAAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References