Detailed information of TA system TA212510

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4183158..4183972
• Replicon: chromosome
• Accession: NZ_CP081008
• Organism: Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K3831_RS20175
• Protein ID: WP_001054376.1
• Coordinates: 4183715..4183972 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K3831_RS20170
• Protein ID: WP_001309181.1
• Coordinates: 4183158..4183703 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3831_RS20140 (4178849)	4178849..4180162	-	1314	WP_000460842.1	PTS sugar transporter subunit IIC SgcC	-
K3831_RS20145 (4180174)	4180174..4180452	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K3831_RS20150 (4180449)	4180449..4181570	-	1122	WP_000010829.1	M42 family metallopeptidase	-
K3831_RS20155 (4181815)	4181815..4181931	-	117	Protein_3992	VOC family protein	-
K3831_RS20160 (4181969)	4181969..4182187	-	219	Protein_3993	hypothetical protein	-
K3831_RS20165 (4182356)	4182356..4183102	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K3831_RS20170 (4183158)	4183158..4183703	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K3831_RS20175 (4183715)	4183715..4183972	-	258	WP_001054376.1	YjhX family toxin	Toxin
K3831_RS23265 (4184463)	4184463..4184594	-	132	WP_001309182.1	hypothetical protein	-
K3831_RS20185 (4184710)	4184710..4185950	+	1241	Protein_3998	helicase YjhR	-
K3831_RS23270 (4186218)	4186218..4186423	-	206	Protein_3999	HNH endonuclease	-
K3831_RS20190 (4186533)	4186533..4187513	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K3831_RS20195 (4187578)	4187578..4188684	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / csgG / csgF / csgE / csgD / csgB / csgA / csgC	4183158..4202982	19824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T212510 WP_001054376.1 NZ_CP081008:c4183972-4183715 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T212510 NZ_CP107241:4218568-4218819 [Xanthomonas hortorum]
ATGGACACCATCACCTACAGCGCCGCGCGTGCCTCTCTGGCCGACACCATGGACCGTGTCGTCAATGACCATGAGCCGGT
GATCATTACACGCAGCGGTGAGCGCGCCGTAGTCATGGTGTCGCTGGAAGACTACAAGGCCATGGAAGAGACCGCTTACC
TGCTGCGCAGCCCGAAGAGCGCGCAACGGCTGCTGGAATCCATTGCCCAGCTTGAGGCCGGTCGCGGCAAGGAACGGGAA
CTGGCTGAGTGA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT212510 WP_001309181.1 NZ_CP081008:c4183703-4183158 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT212510 NZ_CP107241:4218816-4219073 [Xanthomonas hortorum]
GTGATTCTGCAGTTTGCTGACAATGCCTGGGAGGACTACCTCTACTGGCAACAGACCGACAAGAAGATGCTCAAACGCAT
CAACGAGTTGATCAAGGCCATTCAGCGCGATCCTTTCCAGGGCATCGGCAAACCCGAACCGTTGCGCCACGCGCTGGCAG
GCTATTGGTCACGACGCATCAATGACGAACACCGGGTTGTCTACAAGGCCGAGAACGGCATCCTGCTGATTGCTCAGGCG
CGCTACCACTACGCGTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8