Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3784305..3785136 Replicon chromosome
Accession NZ_CP081008
Organism Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K3831_RS18200 Protein ID WP_000854814.1
Coordinates 3784762..3785136 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag K3831_RS18195 Protein ID WP_001285584.1
Coordinates 3784305..3784673 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3831_RS18180 (3781972) 3781972..3783504 + 1533 WP_001350525.1 protein YeeR -
K3831_RS18185 (3783501) 3783501..3783947 + 447 WP_000187523.1 RadC family protein -
K3831_RS18190 (3784010) 3784010..3784231 + 222 WP_000692323.1 DUF987 domain-containing protein -
K3831_RS18195 (3784305) 3784305..3784673 + 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K3831_RS18200 (3784762) 3784762..3785136 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K3831_RS18205 (3785133) 3785133..3785327 + 195 WP_000988600.1 DUF5983 family protein -
K3831_RS18210 (3785373) 3785373..3785453 + 81 Protein_3597 hypothetical protein -
K3831_RS18215 (3785742) 3785742..3785870 - 129 Protein_3598 transposase domain-containing protein -
K3831_RS18220 (3785990) 3785990..3786124 + 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
K3831_RS18225 (3786225) 3786225..3786554 - 330 WP_000450409.1 DUF496 family protein -
K3831_RS18230 (3786726) 3786726..3787784 - 1059 WP_046613327.1 FUSC family protein -
K3831_RS18235 (3787982) 3787982..3788455 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
K3831_RS18240 (3788574) 3788574..3789740 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T212508 WP_000854814.1 NZ_CP081008:3784762-3785136 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T212508 NZ_CP107241:98638-98937 [Xanthomonas hortorum]
ATGGCTGAAATAGTCTGGTCGGAGCCAGCACTGGCTGATTTGGACGTGATTGCCGACTACATCGCAATCGAGGACGCGCT
CGCCGCAGCGACACTGGTGAGACGGGTGTTCGCGCACGTCGAGCAATTGATCGAACACCCGGAGAGTGGCAGCCGCCCCC
AGGAGCTGAAGCGCTCGCGCTATCGCCAGATTGTCGAACCGCCTTGCCGCGTGTTCTACCGCGTGGATGGGCAACGCATC
GTAGTGGTGCACGTGATGCGGTCGGAGCGTTCGCTCCGCAAGAGCCGCCTTTCACGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT212508 WP_001285584.1 NZ_CP081008:3784305-3784673 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT212508 NZ_CP107241:98393-98650 [Xanthomonas hortorum]
ATGCGCACCGAACTTGTCACCACGCTCAAGCGTCAGGCCAGCGAACTCCTTGCAGCTGCAGAGCGCGACAAGGAGCCGAT
CCTGATCACCCAGCATGGTTTGCCCAGCGCTTATCTGGTGGATGTTGCCAGCTATGAGCGCATGCAGCAGCGCATGGAGA
TCCTTGAAGGAATCGCCCGTGGCGAAATGGCAGTAGCGGAAGGCCGCACGCTGAGTCACGAGCAGGCCAAGCAACGCATG
GCGCGATGGCTGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References