Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3046929..3047608 | Replicon | chromosome |
| Accession | NZ_CP081008 | ||
| Organism | Escherichia coli strain XL10-gold-ultracompetent | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | P77692 |
| Locus tag | K3831_RS14485 | Protein ID | WP_000854672.1 |
| Coordinates | 3047267..3047608 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | Q47684 |
| Locus tag | K3831_RS14480 | Protein ID | WP_000070395.1 |
| Coordinates | 3046929..3047246 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K3831_RS14430 (3041976) | 3041976..3042839 | + | 864 | WP_001065553.1 | GTPase family protein | - |
| K3831_RS14435 (3042931) | 3042931..3043752 | + | 822 | WP_000197389.1 | DUF932 domain-containing protein | - |
| K3831_RS14440 (3043969) | 3043969..3044160 | + | 192 | Protein_2853 | DeoR family transcriptional regulator | - |
| K3831_RS14445 (3044207) | 3044207..3044383 | + | 177 | WP_001285112.1 | hypothetical protein | - |
| K3831_RS14450 (3044383) | 3044383..3044826 | + | 444 | WP_000824223.1 | lipoprotein YafY | - |
| K3831_RS14455 (3044849) | 3044849..3045316 | + | 468 | WP_001547765.1 | protein YkfB | - |
| K3831_RS14460 (3045393) | 3045393..3045632 | + | 240 | WP_000194654.1 | DUF905 family protein | - |
| K3831_RS14465 (3045730) | 3045730..3046188 | + | 459 | WP_000211838.1 | antirestriction protein | - |
| K3831_RS14470 (3046204) | 3046204..3046680 | + | 477 | WP_000811693.1 | RadC family protein | - |
| K3831_RS14475 (3046689) | 3046689..3046910 | + | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| K3831_RS14480 (3046929) | 3046929..3047246 | + | 318 | WP_000070395.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| K3831_RS14485 (3047267) | 3047267..3047608 | + | 342 | WP_000854672.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| K3831_RS14495 (3048180) | 3048180..3049433 | - | 1254 | WP_000893278.1 | glutamate-5-semialdehyde dehydrogenase | - |
| K3831_RS14500 (3049445) | 3049445..3050548 | - | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| K3831_RS14505 (3050836) | 3050836..3051891 | + | 1056 | WP_000749863.1 | phosphoporin PhoE | - |
| K3831_RS14510 (3051930) | 3051930..3052331 | - | 402 | WP_000174689.1 | sigma factor-binding protein Crl | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | gmhA/lpcA | 3030180..3076133 | 45953 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12905.01 Da Isoelectric Point: 9.6543
>T212503 WP_000854672.1 NZ_CP081008:3047267-3047608 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T212503 NZ_CP107111:c2069346-2069056 [Clostridioides difficile]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11939.59 Da Isoelectric Point: 6.7391
>AT212503 WP_000070395.1 NZ_CP081008:3046929-3047246 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT212503 NZ_CP107111:c2069621-2069343 [Clostridioides difficile]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|