Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3012526..3013164 Replicon chromosome
Accession NZ_CP081008
Organism Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag K3831_RS14290 Protein ID WP_000813794.1
Coordinates 3012526..3012702 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag K3831_RS14295 Protein ID WP_001270286.1
Coordinates 3012748..3013164 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3831_RS14270 (3008145) 3008145..3009320 - 1176 WP_001236265.1 BenE family transporter YdcO -
K3831_RS14275 (3009412) 3009412..3009948 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
K3831_RS14280 (3010021) 3010021..3011982 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
K3831_RS14285 (3012074) 3012074..3012304 - 231 WP_000494244.1 YncJ family protein -
K3831_RS14290 (3012526) 3012526..3012702 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
K3831_RS14295 (3012748) 3012748..3013164 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
K3831_RS14300 (3013243) 3013243..3014649 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
K3831_RS14305 (3014894) 3014894..3016039 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
K3831_RS14310 (3016057) 3016057..3017070 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
K3831_RS14315 (3017071) 3017071..3018012 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T212502 WP_000813794.1 NZ_CP081008:3012526-3012702 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T212502 NZ_CP107110:c2069511-2069221 [Clostridioides difficile]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT212502 WP_001270286.1 NZ_CP081008:3012748-3013164 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT212502 NZ_CP107110:c2069786-2069508 [Clostridioides difficile]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References