Detailed information of TA system TA212502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3012526..3013164
• Replicon: chromosome
• Accession: NZ_CP081008
• Organism: Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K3831_RS14290
• Protein ID: WP_000813794.1
• Coordinates: 3012526..3012702 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K3831_RS14295
• Protein ID: WP_001270286.1
• Coordinates: 3012748..3013164 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3831_RS14270 (3008145)	3008145..3009320	-	1176	WP_001236265.1	BenE family transporter YdcO	-
K3831_RS14275 (3009412)	3009412..3009948	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K3831_RS14280 (3010021)	3010021..3011982	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K3831_RS14285 (3012074)	3012074..3012304	-	231	WP_000494244.1	YncJ family protein	-
K3831_RS14290 (3012526)	3012526..3012702	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K3831_RS14295 (3012748)	3012748..3013164	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K3831_RS14300 (3013243)	3013243..3014649	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
K3831_RS14305 (3014894)	3014894..3016039	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
K3831_RS14310 (3016057)	3016057..3017070	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
K3831_RS14315 (3017071)	3017071..3018012	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T212502 WP_000813794.1 NZ_CP081008:3012526-3012702 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T212502 NZ_CP107110:c2069511-2069221 [Clostridioides difficile]
ATGAAAGAAACCAAATATACCGTCAAGTACACGACCAGTTTCAAAAAAGACTACAAACGAGCCATCAAGCGTGGCTTGAA
GATCGAACTGCTGGAGCAGGTCGTAGCGTTGCTGGCGATGGGCGAACCGCTGCCGGATAAGAACCGTGACCATGACCTGT
CCGGCGATTGGGCAGGCCATCGGGAATGTCACATTCTCCCGGACTGGCTGCTTGTCTACCGCATAGAAGATGATGTTCTG
GTGCTGACGCTGGCCCGCACCGGCACACACAGCGACTTGTTCGGCAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT212502 WP_001270286.1 NZ_CP081008:3012748-3013164 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT212502 NZ_CP107110:c2069786-2069508 [Clostridioides difficile]
ATGGCTGGAAATACCACAAACATCAGTATCCGCATGGACGCAGATTTGAAAGCGCAGGCGGACGCACTGTTTACTGAACT
TGGCATGAACCTGACTACGGCGTTTAACATCTTTGTGCGCCAGTCGCTTCGTGAAGGCGGCATTCCCTTTGAAGTAAGGC
TGGAACAGCCGAACAAAGAAACGGTTGCTGCCATGCTGGAAGCGGAAAGGATTGCAAAAGACCCGTCTGTAAAGGGCTAC
AATGACCTTGACGAGTTATTTGCTGACCTGAAAAGATGA

Structures

Toxin

• PDB: 6HPB