212498

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 2667233..2668670 Replicon chromosome
Accession NZ_CP081008
Organism Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag K3831_RS12635 Protein ID WP_000155570.1
Coordinates 2667233..2668306 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag K3831_RS12640 Protein ID WP_000461704.1
Coordinates 2668299..2668670 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3831_RS12620 (2662861) 2662861..2664855 - 1995 Protein_2489 helicase-related protein -
K3831_RS12625 (2664852) 2664852..2666468 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
K3831_RS12630 (2666828) 2666828..2667091 - 264 WP_000502050.1 DUF1524 domain-containing protein -
K3831_RS12635 (2667233) 2667233..2668306 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
K3831_RS12640 (2668299) 2668299..2668670 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
K3831_RS12645 (2669025) 2669025..2669888 + 864 WP_000787597.1 GTPase family protein -
K3831_RS12650 (2669980) 2669980..2670801 + 822 WP_000197391.1 DUF932 domain-containing protein -
K3831_RS12655 (2671018) 2671018..2671719 + 702 WP_000189409.1 WYL domain-containing protein -
K3831_RS12660 (2671760) 2671760..2671996 + 237 WP_001144029.1 protein YpjK -
K3831_RS12665 (2671996) 2671996..2672439 + 444 WP_000824222.1 lipoprotein YfjS -
K3831_RS12670 (2672463) 2672463..2672930 + 468 WP_000702495.1 protein YfjT -
K3831_RS23140 (2673317) 2673317..2673469 - 153 Protein_2500 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T212498 WP_000155570.1 NZ_CP081008:2667233-2668306 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T212498 NZ_CP107079:480138-480488 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT212498 WP_000461704.1 NZ_CP081008:2668299-2668670 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT212498 NZ_CP107079:479851-480132 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References