Detailed information of TA system TA212486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1111107..1111725
• Replicon: chromosome
• Accession: NZ_CP081008
• Organism: Escherichia coli strain XL10-gold-ultracompetent

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: K3831_RS05270
• Protein ID: WP_001290581.1
• Coordinates: 1111107..1111325 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: K3831_RS05275
• Protein ID: WP_000344800.1
• Coordinates: 1111351..1111725 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3831_RS05235 (1106396)	1106396..1106968	+	573	WP_000779842.1	YbaY family lipoprotein	-
K3831_RS05240 (1106999)	1106999..1107310	-	312	WP_000409911.1	MGMT family protein	-
K3831_RS05250 (1107689)	1107689..1108042	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
K3831_RS05255 (1108084)	1108084..1109634	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
K3831_RS05260 (1109798)	1109798..1110268	-	471	WP_000136192.1	YlaC family protein	-
K3831_RS05265 (1110384)	1110384..1110935	-	552	WP_000102564.1	maltose O-acetyltransferase	-
K3831_RS05270 (1111107)	1111107..1111325	-	219	WP_001290581.1	HHA domain-containing protein	Toxin
K3831_RS05275 (1111351)	1111351..1111725	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
K3831_RS05280 (1112271)	1112271..1115420	-	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
K3831_RS05285 (1115443)	1115443..1116636	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T212486 WP_001290581.1 NZ_CP081008:c1111325-1111107 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T212486 NZ_CP107064:c6095064-6094606 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAATGACCCGGCGGGCTTCGAGTCCAATGCCAACGTCAAGCTCTTCCGGGCCCTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAACACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGACGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT212486 WP_000344800.1 NZ_CP081008:c1111725-1111351 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT212486 NZ_CP107064:c6095381-6095064 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGCTGACCTCTAAAGGCCAGATCACGCTGCCCAAGCCCATCCGGCAGGCGCTCGG
CGTCGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGTGTTGATGCCGAGCACGAGG
ACCCCGCCATTGGCGCGTTTCTGAGCCTGCTGGCCCGCGACATCGAAGCTGGCCGGAACGTCCGGGGCCTGCCCGAGGAT
CTGGCGCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5