Detailed information of TA system TA212469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4181376..4182190
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K3826_RS20130
• Protein ID: WP_001054376.1
• Coordinates: 4181933..4182190 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K3826_RS20125
• Protein ID: WP_001309181.1
• Coordinates: 4181376..4181921 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS20095 (4177067)	4177067..4178380	-	1314	WP_000460842.1	PTS sugar transporter subunit IIC SgcC	-
K3826_RS20100 (4178392)	4178392..4178670	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K3826_RS20105 (4178667)	4178667..4179788	-	1122	WP_000010829.1	M42 family metallopeptidase	-
K3826_RS20110 (4180033)	4180033..4180149	-	117	Protein_3949	VOC family protein	-
K3826_RS20115 (4180187)	4180187..4180405	-	219	Protein_3950	hypothetical protein	-
K3826_RS20120 (4180574)	4180574..4181320	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K3826_RS20125 (4181376)	4181376..4181921	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K3826_RS20130 (4181933)	4181933..4182190	-	258	WP_001054376.1	YjhX family toxin	Toxin
K3826_RS22840 (4182681)	4182681..4182812	-	132	WP_001309182.1	hypothetical protein	-
K3826_RS20140 (4182928)	4182928..4184168	+	1241	Protein_3955	helicase YjhR	-
K3826_RS22845 (4184436)	4184436..4184641	-	206	Protein_3956	HNH endonuclease	-
K3826_RS20145 (4184751)	4184751..4185731	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
K3826_RS20150 (4185796)	4185796..4186902	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T212469 WP_001054376.1 NZ_CP081007:c4182190-4181933 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T212469 NZ_CP107043:c2231199-2230915 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT212469 WP_001309181.1 NZ_CP081007:c4181921-4181376 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT212469 NZ_CP107043:c2231431-2231189 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8