Detailed information of TA system TA212468

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4092561..4093186
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3826_RS19620
• Protein ID: WP_000911324.1
• Coordinates: 4092788..4093186 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: K3826_RS19615
• Protein ID: WP_000450532.1
• Coordinates: 4092561..4092788 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS19615 (4092561)	4092561..4092788	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K3826_RS19620 (4092788)	4092788..4093186	+	399	WP_000911324.1	type II toxin-antitoxin system VapC family toxin	Toxin
K3826_RS19625 (4093195)	4093195..4095348	-	2154	WP_000009350.1	type IV conjugative transfer system coupling protein TraD	-
K3826_RS19630 (4095601)	4095601..4096332	-	732	WP_000850422.1	conjugal transfer complement resistance protein TraT	-
K3826_RS19635 (4096357)	4096357..4096878	-	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4057288..4164989	107701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T212468 WP_000911324.1 NZ_CP081007:4092788-4093186 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T212468 NZ_CP107043:c2112547-2112014 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT212468 WP_000450532.1 NZ_CP081007:4092561-4092788 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT212468 NZ_CP107043:c2112824-2112558 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5