Detailed information of TA system TA212465

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 4055819..4056498
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: K3826_RS19435
• Protein ID: WP_000854672.1
• Coordinates: 4056157..4056498 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: K3826_RS19430
• Protein ID: WP_000070395.1
• Coordinates: 4055819..4056136 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS19380 (4050866)	4050866..4051729	+	864	WP_001065553.1	GTPase family protein	-
K3826_RS19385 (4051821)	4051821..4052642	+	822	WP_000197389.1	DUF932 domain-containing protein	-
K3826_RS19390 (4052859)	4052859..4053050	+	192	Protein_3805	DeoR family transcriptional regulator	-
K3826_RS19395 (4053097)	4053097..4053273	+	177	WP_001285112.1	hypothetical protein	-
K3826_RS19400 (4053273)	4053273..4053716	+	444	WP_000824223.1	lipoprotein YafY	-
K3826_RS19405 (4053739)	4053739..4054206	+	468	WP_001547765.1	protein YkfB	-
K3826_RS19410 (4054283)	4054283..4054522	+	240	WP_000194654.1	DUF905 family protein	-
K3826_RS19415 (4054620)	4054620..4055078	+	459	WP_000211838.1	antirestriction protein	-
K3826_RS19420 (4055094)	4055094..4055570	+	477	WP_000811693.1	RadC family protein	-
K3826_RS19425 (4055579)	4055579..4055800	+	222	WP_000691994.1	DUF987 domain-containing protein	-
K3826_RS19430 (4055819)	4055819..4056136	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
K3826_RS19435 (4056157)	4056157..4056498	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
K3826_RS19445 (4057070)	4057070..4058323	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
K3826_RS19450 (4058335)	4058335..4059438	-	1104	WP_001285288.1	glutamate 5-kinase	-
K3826_RS19455 (4059726)	4059726..4060781	+	1056	WP_000749863.1	phosphoporin PhoE	-
K3826_RS19460 (4060820)	4060820..4061221	-	402	WP_000174689.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T212465 WP_000854672.1 NZ_CP081007:4056157-4056498 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T212465 NZ_CP107041:162669-163994 [Klebsiella oxytoca]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTGCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGCATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGGCCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCCTGGCAGAAGTGCAAATTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCAGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATACAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT212465 WP_000070395.1 NZ_CP081007:4055819-4056136 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT212465 NZ_CP107041:162371-162664 [Klebsiella oxytoca]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGCTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGCGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCATCCTGGGCGTAAAAATCAGCTTCTCCTCGGCAACCACCTCTTCAGAGAGGAAG
CAGACGGAAGAAATGCTTAAATCAAACTCACCTGCACGACAGGAGGATTGGTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684