Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3439172..3439810 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag K3826_RS16270 Protein ID WP_000813794.1
Coordinates 3439172..3439348 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag K3826_RS16275 Protein ID WP_001270286.1
Coordinates 3439394..3439810 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3826_RS16250 (3434791) 3434791..3436005 - 1215 WP_001326689.1 BenE family transporter YdcO -
K3826_RS16255 (3436058) 3436058..3436594 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
K3826_RS16260 (3436667) 3436667..3438628 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
K3826_RS16265 (3438720) 3438720..3438950 - 231 WP_000494244.1 YncJ family protein -
K3826_RS16270 (3439172) 3439172..3439348 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
K3826_RS16275 (3439394) 3439394..3439810 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
K3826_RS16280 (3439889) 3439889..3441295 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
K3826_RS16285 (3441540) 3441540..3442685 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
K3826_RS16290 (3442703) 3442703..3443716 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
K3826_RS16295 (3443717) 3443717..3444658 + 942 WP_001251304.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T212463 WP_000813794.1 NZ_CP081007:3439172-3439348 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T212463 NZ_CP107040:c4651464-4651207 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGACTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT212463 WP_001270286.1 NZ_CP081007:3439394-3439810 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT212463 NZ_CP107040:c4651196-4650645 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGATCTGGTTGCCGCTTTGCTCAACGATGAAAGTGCTCACCCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCAGTTGGGCATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCGCTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References