Detailed information of TA system TA212463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3439172..3439810
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K3826_RS16270
• Protein ID: WP_000813794.1
• Coordinates: 3439172..3439348 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K3826_RS16275
• Protein ID: WP_001270286.1
• Coordinates: 3439394..3439810 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS16250 (3434791)	3434791..3436005	-	1215	WP_001326689.1	BenE family transporter YdcO	-
K3826_RS16255 (3436058)	3436058..3436594	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K3826_RS16260 (3436667)	3436667..3438628	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K3826_RS16265 (3438720)	3438720..3438950	-	231	WP_000494244.1	YncJ family protein	-
K3826_RS16270 (3439172)	3439172..3439348	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K3826_RS16275 (3439394)	3439394..3439810	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K3826_RS16280 (3439889)	3439889..3441295	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
K3826_RS16285 (3441540)	3441540..3442685	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
K3826_RS16290 (3442703)	3442703..3443716	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
K3826_RS16295 (3443717)	3443717..3444658	+	942	WP_001251304.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T212463 WP_000813794.1 NZ_CP081007:3439172-3439348 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T212463 NZ_CP107040:c4651464-4651207 [Klebsiella oxytoca]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCATATTCGCGATGCATC
AGGCCGCGTAACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAACTCATTAAGTCCGTCAACGGGCAGCCCTACCGTATCAATACCACCGGACTGAACAACGTTCGCGCT
CAGCTGGACAACCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT212463 WP_001270286.1 NZ_CP081007:3439394-3439810 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT212463 NZ_CP107040:c4651196-4650645 [Klebsiella oxytoca]
ATGACAAACCATAACTTCACCTTTCACATCACCTCAGAGCGTGATGCTGATGATATTCGCGAAGTTGAAACCCGGGCCTT
CGGCTTTAGCAAAGAGGCCGATCTGGTTGCCGCTTTGCTCAACGATGAAAGTGCTCACCCGAGCTTATCTCTGCTGGCCA
AACATAATGGAAAAGCAGTTGGGCATATTTTGTTCACCCTGGCGACGTTCAAAGGGGAGTCTGACTCTCCGATGATGCAT
ATCCTTGCCCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTAGGCGGATTGCTTATCCAGCGCGGTATTGAGCA
TCTGAAAGCCGCAGGAAGCGAGGCGGTATTTGTTCTGGGACATGCCGCTTATTACCCGCGGCATGGCTTTGAACCCTGTG
CCGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGAGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCC
CGGCCGCTGGGGAGAACCGGTCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• PDB: 6HPB