Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3248794..3249291 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K3826_RS15435 Protein ID WP_001173343.1
Coordinates 3248794..3248967 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K3826_RS15440 Protein ID WP_000398619.1
Coordinates 3248995..3249291 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3826_RS15425 (3245464) 3245464..3246921 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
K3826_RS15430 (3247158) 3247158..3248675 + 1518 WP_001295074.1 lysine--tRNA ligase -
K3826_RS15435 (3248794) 3248794..3248967 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K3826_RS15440 (3248995) 3248995..3249291 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K3826_RS15445 (3249518) 3249518..3249790 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
K3826_RS15450 (3249802) 3249802..3250032 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
K3826_RS15455 (3250213) 3250213..3251844 + 1632 WP_001216456.1 sensor histidine kinase -
K3826_RS15460 (3251841) 3251841..3252560 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T212460 WP_001173343.1 NZ_CP081007:c3248967-3248794 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T212460 NZ_CP107040:c1354182-1353814 [Klebsiella oxytoca]
ATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCAAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAACACATTGATGCGG
GAATCTCACTGTGTGACGCTGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCCTTGATATGCTCCGAGACCGGAAGGCTACCGGGCTGATGACCCGCAAGGGATA
TAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT212460 WP_000398619.1 NZ_CP081007:c3249291-3248995 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT212460 NZ_CP107040:c1354586-1354227 [Klebsiella oxytoca]
ATGCAGAAAGCAACCCGTGTAATCAAACATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACGTCACGCCATGTTT
TGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCACCACCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGTTACTGATGAAGCAGATGGAGCTCATGCTGACCAGCAGCGAACTCATG
CCCCGCATTCAGCGCTGCATCACGCTCCATGCAAAAGGGCTGATATGTAAGGCCGATACGCTCGGTTCCTGTGGCTATTT
GTATATCGTAATTTATCCGGCGTCTGCAACAACCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References