Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2568583..2569250 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag K3826_RS12090 Protein ID WP_001094400.1
Coordinates 2568921..2569250 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag K3826_RS12085 Protein ID WP_000072690.1
Coordinates 2568583..2568900 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3826_RS12055 (2563635) 2563635..2564644 - 1010 Protein_2372 arsenic transporter -
K3826_RS12060 (2564786) 2564786..2566489 + 1704 WP_000896263.1 protein YfjW -
K3826_RS12065 (2567061) 2567061..2567284 + 224 Protein_2374 DUF905 domain-containing protein -
K3826_RS12070 (2567387) 2567387..2567845 + 459 WP_000211841.1 antirestriction protein -
K3826_RS12075 (2567854) 2567854..2568336 + 483 WP_001407480.1 RadC family protein -
K3826_RS12080 (2568345) 2568345..2568545 + 201 WP_001393456.1 DUF987 domain-containing protein -
K3826_RS12085 (2568583) 2568583..2568900 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K3826_RS12090 (2568921) 2568921..2569250 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
K3826_RS12095 (2569614) 2569614..2574194 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T212445 WP_001094400.1 NZ_CP081007:2568921-2569250 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T212445 NZ_CP107029:621467-621649 [Pseudomonas aeruginosa]
ATGAAGTACAGCGAGTTTCGGCGCTGGTTGAAGGCTCGGGGAGTCATTTTCGAGCCTGCCAAGGGAAGCCATTTCAAGGT
TTACTACGGCGACAACCAGACGATCTTCCCCGACCATGGAGCCAAGGAAATAGGCGATGGGTTGAGGAAGAAGATAATCA
AAGACCTGGGCCTCAAGGATTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT212445 WP_000072690.1 NZ_CP081007:2568583-2568900 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT212445 NZ_CP107029:621682-622104 [Pseudomonas aeruginosa]
ATGTTTGACTATCCCGTAACGGTTCACGAGGAAGCCGGCAGCGTGTGGGTCAGCTGCGACGATGTACCCGAAATGGCGAG
CGCCGGAGATACCGTTGATGAAGCGCTGCTGGACGCTGTGGAAGGCCTAGAGTCGGCCCTGTCGCTCTATGTGGACCGGC
GGCAGTCGATCCCACTGCCGTCGAAAGGCAAAGCGGGTCAGTCGATCGTTCGATTGCCCGCGCTCACTTCGGCCAAGATT
GCGCTGTGGAACACCATGCTGGCTCAGAACGTAGGGAAGGCGGAACTGGCTCGCCGGCTGGGAGTGAATCGCGTCCAGGT
AGACCGCCTGGTGGATCTGCTCCACGGGTCGAAGATCGAAGCGGTGGAACATGCTCTGGCGATCTTGGGCCAACGGTTGG
CTGTGACTGTCATTGCGGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References