Detailed information of TA system TA212444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 2557386..2558823
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: K3826_RS12010
• Protein ID: WP_000155570.1
• Coordinates: 2557386..2558459 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: K3826_RS12015
• Protein ID: WP_000461704.1
• Coordinates: 2558452..2558823 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS11995 (2552819)	2552819..2555008	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
K3826_RS12000 (2555005)	2555005..2556621	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
K3826_RS12005 (2556981)	2556981..2557244	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
K3826_RS12010 (2557386)	2557386..2558459	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
K3826_RS12015 (2558452)	2558452..2558823	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
K3826_RS12020 (2559178)	2559178..2560041	+	864	WP_000787597.1	GTPase family protein	-
K3826_RS12025 (2560133)	2560133..2560954	+	822	WP_000197391.1	DUF932 domain-containing protein	-
K3826_RS12030 (2561171)	2561171..2561872	+	702	WP_000189409.1	WYL domain-containing protein	-
K3826_RS12035 (2561913)	2561913..2562149	+	237	WP_001144029.1	protein YpjK	-
K3826_RS12040 (2562149)	2562149..2562592	+	444	WP_000824222.1	lipoprotein YfjS	-
K3826_RS12045 (2562616)	2562616..2563083	+	468	WP_000702495.1	protein YfjT	-
K3826_RS22785 (2563470)	2563470..2563622	-	153	Protein_2371	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T212444 WP_000155570.1 NZ_CP081007:2557386-2558459 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T212444 NZ_CP107029:c141924-141643 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT212444 WP_000461704.1 NZ_CP081007:2558452-2558823 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT212444 NZ_CP107029:c142148-141921 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4