Detailed information of TA system TA212443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1848832..1849450
• Replicon: chromosome
• Accession: NZ_CP081007
• Organism: Escherichia coli strain XL1-Blue

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: K3826_RS08700
• Protein ID: WP_001290581.1
• Coordinates: 1848832..1849050 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: K3826_RS08705
• Protein ID: WP_000344800.1
• Coordinates: 1849076..1849450 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3826_RS08665 (1844121)	1844121..1844693	+	573	WP_000779842.1	YbaY family lipoprotein	-
K3826_RS08670 (1844724)	1844724..1845035	-	312	WP_000409911.1	MGMT family protein	-
K3826_RS08680 (1845414)	1845414..1845767	+	354	WP_000878140.1	DUF1428 domain-containing protein	-
K3826_RS08685 (1845809)	1845809..1847359	-	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
K3826_RS08690 (1847523)	1847523..1847993	-	471	WP_000136192.1	YlaC family protein	-
K3826_RS08695 (1848109)	1848109..1848660	-	552	WP_000102564.1	maltose O-acetyltransferase	-
K3826_RS08700 (1848832)	1848832..1849050	-	219	WP_001290581.1	HHA domain-containing protein	Toxin
K3826_RS08705 (1849076)	1849076..1849450	-	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
K3826_RS08710 (1849996)	1849996..1853145	-	3150	WP_001132469.1	efflux RND transporter permease AcrB	-
K3826_RS08715 (1853168)	1853168..1854361	-	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T212443 WP_001290581.1 NZ_CP081007:c1849050-1848832 [Escherichia coli]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T212443 NZ_CP107027:c2584833-2584483 [Cytobacillus firmus]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGTTCAGAACAAGGCGGCGTCCGTCCAGT
GCTTGTCATCCAAAACGACATCGGGAATCGGTTTAGTCCCACAGTAATTGTTGCAGCAATCACAGCTCAGATTCAAAAAG
CAAAGCTGCCTACTCACGTTGAAATTGATGCGAAGCGTTATGGTTTTGAAAGGGATTCGGTCATCTTATTAGAACAGATT
CGTACAATTGATAAACAACGCTTAACCGATAAAATAACCCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCCGT
GCAAATCAGCTTAGGCCTCATCGAATTTTAG

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT212443 WP_000344800.1 NZ_CP081007:c1849450-1849076 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT212443 NZ_CP107027:c2585119-2584838 [Cytobacillus firmus]
GTGTCTGAGTCCAGCACAACAACAGAGATAATGGTGAAATTACCGCAGCATCTTTTAACCGAGTTAGACGGATTTGCTAA
ACAGGAAAACGTTAACCGGAGCGAATTCATTTATCAAGCAACCAAAATGTATTTGCGTGAACGCAAAAAGAGACAAATTC
GCGAGTCCATGAGACGTGGTTACATGGAGATGGCGAAAATAAATTTGACGATTGCATCTGAAGCATTTCAAGCGGAATAC
GAGGCAGAACACACAGTTGAACGTCTAGTTAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5