Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1717592..1718246 Replicon chromosome
Accession NZ_CP081007
Organism Escherichia coli strain XL1-Blue

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K3826_RS08085 Protein ID WP_000244777.1
Coordinates 1717839..1718246 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K3826_RS08080 Protein ID WP_000354046.1
Coordinates 1717592..1717858 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3826_RS08055 (1712761) 1712761..1713504 + 744 WP_000951964.1 SDR family oxidoreductase -
K3826_RS08060 (1713561) 1713561..1714994 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
K3826_RS08065 (1715039) 1715039..1715350 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
K3826_RS08070 (1715514) 1715514..1716173 + 660 WP_000250274.1 hemolysin III family protein -
K3826_RS08075 (1716369) 1716369..1717349 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K3826_RS08080 (1717592) 1717592..1717858 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K3826_RS08085 (1717839) 1717839..1718246 + 408 WP_000244777.1 protein YgfX Toxin
K3826_RS08090 (1718286) 1718286..1718807 - 522 WP_001055874.1 flavodoxin FldB -
K3826_RS08095 (1718919) 1718919..1719815 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K3826_RS08100 (1719840) 1719840..1720550 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K3826_RS08105 (1720556) 1720556..1722289 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T212442 WP_000244777.1 NZ_CP081007:1717839-1718246 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T212442 NZ_CP107020:c2710131-2709865 [Brachybacterium huguangmaarense]
ATGAAGACGCTCAGCTACACGGAATCTCGCGCACGGTACGCCGAGGTCCTGGACGGCGTCGTCGCGGACCGTGAGGAAGT
GGTCATCACGCGCGCCGGGCACGAGCCCGTCGTCATCGTGTCCCTCGACGACTACGAGTCCCTGCGCGAGACGGCGTATC
TGATGCGGTCGCCCGCCAACGCCCGCCGCCTGCTGGATGCCATGGAGCAGCTCGAGGGCGGCCGCGGGGCGCGTCACGAG
CTCGTCGACGTCGATGGCGACGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT212442 WP_000354046.1 NZ_CP081007:1717592-1717858 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT212442 NZ_CP107020:c2709863-2709606 [Brachybacterium huguangmaarense]
GTGCTCCTCGTCTGGAGCTCGAGCGCCTGGGACGACTACCTCTGGTGGCAGTCGCAGGATCGCAAGACCCTGCGGCGGAT
CAACACGCTCATCCAGGACATCTCCCGCAACGGCCACGACGGCATCGGCAAGCCCGAGGCCCTGAAGCATGACTTCGCGG
GCTATTGGTCCCGCCGCATCACCGACGAGCACCGCCTCGTCTACAAGATCGAAGGCGACGAGGTGCGCATCGCGGCGTGC
CGGTACCACTACCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References