Detailed information of TA system TA212438

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2451916..2452596
• Replicon: chromosome
• Accession: NZ_CP081006
• Organism: Mycobacterium sp. IDR2000157661

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: K3G64_RS13080
• Protein ID: WP_238950310.1
• Coordinates: 2452159..2452596 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: -
• Locus tag: K3G64_RS13075
• Protein ID: WP_238950309.1
• Coordinates: 2451916..2452155 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3G64_RS13065 (K3G64_12985)	2447911..2449518	+	1608	WP_238950308.1	serine hydrolase	-
K3G64_RS13070 (K3G64_12990)	2449521..2451902	+	2382	WP_238950636.1	cation-translocating P-type ATPase	-
K3G64_RS13075 (K3G64_12995)	2451916..2452155	+	240	WP_238950309.1	antitoxin	Antitoxin
K3G64_RS13080 (K3G64_13000)	2452159..2452596	+	438	WP_238950310.1	SRPBCC family protein	Toxin
K3G64_RS13085 (K3G64_13005)	2452602..2454005	-	1404	WP_238950311.1	HNH endonuclease signature motif containing protein	-
K3G64_RS13090 (K3G64_13010)	2454158..2455615	-	1458	WP_238950312.1	long-chain fatty acid--CoA ligase	-
K3G64_RS13095 (K3G64_13015)	2455612..2456385	-	774	WP_238950313.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15657.25 Da
• Isoelectric Point: 10.1670

>T212438 WP_238950310.1 NZ_CP081006:2452159-2452596 [Mycobacterium sp. IDR2000157661]
MAKLSVSVDVPLPPEQAWAAASDLSRYKEWLSIHRVWRSKLPETIEKGTVVESIVEVKGMPNRIRWTVVHYKPPQAMTLN
GEGVGGVKVKLIGKIAPSGDASSVTFDVHLGGPALFGPIGMVVAAALKGDIRESLERFRSVFAPA

Nucleotide

• Length: 438 bp

>T212438 NZ_CP107018:4024-4338 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8506.61 Da
• Isoelectric Point: 11.0749

>AT212438 WP_238950309.1 NZ_CP081006:2451916-2452155 [Mycobacterium sp. IDR2000157661]
MAIFDKVKNVLAKNADKANTAIDKAGDLVDKRTRGKYAQQVNKVQDAARKAVADNAGRSGQQPPRQQPPRQPPTAPPTS

Nucleotide

• Length: 240 bp

>AT212438 NZ_CP107018:3758-4036 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA