Detailed information of TA system TA212437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 1665401..1666095
• Replicon: chromosome
• Accession: NZ_CP081006
• Organism: Mycobacterium sp. IDR2000157661

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3G64_RS09015
• Protein ID: WP_238950532.1
• Coordinates: 1665401..1665760 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K3G64_RS09020
• Protein ID: WP_238949255.1
• Coordinates: 1665811..1666095 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3G64_RS08995 (K3G64_08935)	1660543..1661709	+	1167	WP_238949248.1	MFS transporter	-
K3G64_RS09000 (K3G64_08940)	1661722..1663191	-	1470	WP_238949251.1	APC family permease	-
K3G64_RS09005 (K3G64_08945)	1663393..1664163	+	771	WP_238949252.1	DUF1906 domain-containing protein	-
K3G64_RS09010 (K3G64_08950)	1664176..1665198	-	1023	WP_238949253.1	glycoside hydrolase	-
K3G64_RS09015 (K3G64_08955)	1665401..1665760	-	360	WP_238950532.1	VapC toxin family PIN domain ribonuclease	Toxin
K3G64_RS09020 (K3G64_08960)	1665811..1666095	-	285	WP_238949255.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
K3G64_RS09030 (K3G64_08965)	1666533..1666976	+	444	WP_238949256.1	DUF4174 domain-containing protein	-
K3G64_RS09035 (K3G64_08970)	1667132..1667650	+	519	WP_238949258.1	CIA30 family protein	-
K3G64_RS09040 (K3G64_08975)	1667680..1670226	+	2547	WP_238950534.1	DEAD/DEAH box helicase	-
K3G64_RS09045 (K3G64_08980)	1670306..1670977	-	672	WP_238949259.1	L,D-transpeptidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (25-111)

Antitoxin

• Post-segregation antitoxin CcdA (25-68)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13125.06 Da
• Isoelectric Point: 5.0953

>T212437 WP_238950532.1 NZ_CP081006:c1665760-1665401 [Mycobacterium sp. IDR2000157661]
MVDLLARTDDRYPAVRARLANTVMHAPAHFDAEVLSALGRMQRAGVLTVAQVDVALSELSQAPMTRHDLAPLLVGAWARR
ETLRLTDAFYVELADTADFVLLTTDQRLARAWPLADAID

Nucleotide

• Length: 360 bp

>T212437 NZ_CP107017:95134-95283 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10203.49 Da
• Isoelectric Point: 4.8231

>AT212437 WP_238949255.1 NZ_CP081006:c1666095-1665811 [Mycobacterium sp. IDR2000157661]
MRGPDRTRRLGSTYASVCILVCMARLNVYVPDELAERARALGLNISALTQAAISAELERSGTEAWLDRLEARGTGARHDD
VLDAIDAARDEFGA

Nucleotide

• Length: 285 bp

>AT212437 NZ_CP107017:c95089-95030 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT