Detailed information of TA system TA212436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1648128..1648819
• Replicon: chromosome
• Accession: NZ_CP081006
• Organism: Mycobacterium sp. IDR2000157661

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3G64_RS08940
• Protein ID: WP_238949223.1
• Coordinates: 1648379..1648819 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K3G64_RS08935
• Protein ID: WP_238949219.1
• Coordinates: 1648128..1648382 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3G64_RS08915 (K3G64_08855)	1643566..1644390	-	825	WP_238949214.1	TetR family transcriptional regulator	-
K3G64_RS08920 (K3G64_08860)	1644510..1645607	+	1098	WP_238949216.1	ferredoxin reductase	-
K3G64_RS08925 (K3G64_08865)	1645624..1647018	+	1395	WP_238949217.1	acyl-CoA desaturase	-
K3G64_RS08930 (K3G64_08870)	1647055..1648002	-	948	WP_238950530.1	glycoside hydrolase family 6 protein	-
K3G64_RS08935 (K3G64_08875)	1648128..1648382	+	255	WP_238949219.1	antitoxin	Antitoxin
K3G64_RS08940 (K3G64_08880)	1648379..1648819	+	441	WP_238949223.1	type II toxin-antitoxin system VapC family toxin	Toxin
K3G64_RS08945 (K3G64_08885)	1648906..1649148	+	243	WP_238949225.1	hypothetical protein	-
K3G64_RS08950 (K3G64_08890)	1649243..1650271	+	1029	WP_238949227.1	fused (3R)-hydroxyacyl-ACP dehydratase subunits HadA/HadB	-
K3G64_RS08955 (K3G64_08895)	1650445..1651833	+	1389	WP_238949228.1	wax ester/triacylglycerol synthase family O-acyltransferase	-
K3G64_RS08960 (K3G64_08900)	1651830..1652486	-	657	WP_238950531.1	DsbA family protein	-
K3G64_RS08965 (K3G64_08905)	1652573..1653709	-	1137	WP_238949235.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 15575.63 Da
• Isoelectric Point: 5.4627

>T212436 WP_238949223.1 NZ_CP081006:1648379-1648819 [Mycobacterium sp. IDR2000157661]
VKVVDASILLYSVNSASEHHEACREWLDGALSGSDTVGLAWVPLLAFVRLSTKVGLFPSPLPPSEAMRQVVDWSTAPGAT
VIAPTPRHGDILHGLLANVGTGGNLVNDAHLAALTIEHRASIVSYDNDFGRFDGIRWDTPDALLAR

Nucleotide

• Length: 441 bp

>T212436 NZ_CP107017:c45951-45535 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9438.72 Da
• Isoelectric Point: 10.9716

>AT212436 WP_238949219.1 NZ_CP081006:1648128-1648382 [Mycobacterium sp. IDR2000157661]
MRTTVTLDDDTLALIRRRMREQGVSFKTALNDAIRDGAFGRPPATRFVTRSADLGVPTVNLDRALQIAGELEDEELLRRQ
RRGA

Nucleotide

• Length: 255 bp

>AT212436 NZ_CP107017:c46178-45948 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA