Detailed information of TA system TA212435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 866846..867486
• Replicon: chromosome
• Accession: NZ_CP081006
• Organism: Mycobacterium sp. IDR2000157661

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3G64_RS05020
• Protein ID: WP_238889412.1
• Coordinates: 866846..867232 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K3G64_RS05025
• Protein ID: WP_238889413.1
• Coordinates: 867232..867486 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3G64_RS04995 (K3G64_04960)	862260..863993	-	1734	WP_238889408.1	DUF4407 domain-containing protein	-
K3G64_RS05000 (K3G64_04965)	864171..864566	-	396	WP_238889409.1	PIN domain-containing protein	-
K3G64_RS05005 (K3G64_04970)	864563..864790	-	228	WP_238889410.1	antitoxin	-
K3G64_RS05010 (K3G64_04975)	864873..866492	+	1620	WP_238889411.1	peptide chain release factor 3	-
K3G64_RS05015 (K3G64_04980)	866521..866730	-	210	Protein_804	PIN domain-containing protein	-
K3G64_RS05020 (K3G64_04985)	866846..867232	-	387	WP_238889412.1	type II toxin-antitoxin system VapC family toxin	Toxin
K3G64_RS05025 (K3G64_04990)	867232..867486	-	255	WP_238889413.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K3G64_RS05030 (K3G64_04995)	867644..868795	-	1152	WP_238889414.1	serine hydrolase domain-containing protein	-
K3G64_RS05035 (K3G64_05000)	869104..869641	+	538	Protein_808	glutamate synthase-related protein	-
K3G64_RS05040 (K3G64_05005)	869735..869890	-	156	WP_238889415.1	hypothetical protein	-
K3G64_RS05045 (K3G64_05010)	870526..871524	-	999	WP_238889416.1	AAA family ATPase	-
K3G64_RS05050 (K3G64_05015)	871521..872183	-	663	WP_238889417.1	nicotinamide riboside transporter PnuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	864563..887605	23042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13862.59 Da
• Isoelectric Point: 4.2876

>T212435 WP_238889412.1 NZ_CP081006:c867232-866846 [Mycobacterium sp. IDR2000157661]
MVIDTSALVAMLSDEPEADVFEAAVEADGTRLMSTASYLETAIVIETRFGEPGGRELDLWLHRAAVELVAVDAGQVEVAR
VAYRTYGKGRHRAALNYGDCFSYALAKVSNQPLLFKGDDFAQTDIGGF

Nucleotide

• Length: 387 bp

>T212435 NZ_CP107016:123273-123560 [Klebsiella pneumoniae]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9284.59 Da
• Isoelectric Point: 6.3498

>AT212435 WP_238889413.1 NZ_CP081006:c867486-867232 [Mycobacterium sp. IDR2000157661]
MALSIKDPEADRLARELSARTGESLTEAVLVALRERLARETGRTRSIPLREELAAIRRRCAALPVVDNRSADEILGYDDR
GLPA

Nucleotide

• Length: 255 bp

>AT212435 NZ_CP107016:123025-123276 [Klebsiella pneumoniae]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA