Detailed information of TA system TA212433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 124556..125172
• Replicon: plasmid p20-157661
• Accession: NZ_CP081005
• Organism: Mycobacterium sp. IDR2000157661

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K3G64_RS00600
• Protein ID: WP_238884887.1
• Coordinates: 124556..124903 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K3G64_RS00605
• Protein ID: WP_238884726.1
• Coordinates: 124906..125172 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3G64_RS00580 (K3G64_00580)	120973..121704	+	732	WP_238884719.1	hypothetical protein	-
K3G64_RS00585 (K3G64_00585)	121809..122570	-	762	WP_238884720.1	hypothetical protein	-
K3G64_RS00590 (K3G64_00595)	123073..123735	+	663	WP_238884723.1	Fic family protein	-
K3G64_RS00595 (K3G64_00600)	123790..124491	-	702	WP_238884725.1	hypothetical protein	-
K3G64_RS00600 (K3G64_00605)	124556..124903	-	348	WP_238884887.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K3G64_RS00605 (K3G64_00610)	124906..125172	-	267	WP_238884726.1	antitoxin	Antitoxin
K3G64_RS00610 (K3G64_00615)	125914..126165	+	252	WP_238884728.1	helix-turn-helix domain-containing protein	-
K3G64_RS00615 (K3G64_00620)	126448..127947	+	1500	WP_238884730.1	IS21 family transposase	-
K3G64_RS00620 (K3G64_00625)	127944..128741	+	798	Protein_123	IS21-like element helper ATPase IstB	-
K3G64_RS00625 (K3G64_00630)	129198..129551	-	354	WP_011891521.1	DUF732 domain-containing protein	-
K3G64_RS00630 (K3G64_00635)	129563..130051	-	489	WP_011891522.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..191242	191242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12316.01 Da
• Isoelectric Point: 9.9859

>T212433 WP_238884887.1 NZ_CP081005:c124903-124556 [Mycobacterium sp. IDR2000157661]
VTPARGQVYRCDLGYGAKPWLIVSNNQRNRHTADVVAIRLTTTSRRLPTWVALASSDPLTGYANADNIETLAKDELGDYL
GALSPATMARVNTALAIALALPAGRSPRPQPPPTH

Nucleotide

• Length: 348 bp

>T212433 NZ_CP107015:123110-123421 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9917.91 Da
• Isoelectric Point: 6.7173

>AT212433 WP_238884726.1 NZ_CP081005:c125172-124906 [Mycobacterium sp. IDR2000157661]
MAEEYSTRLRLDASTHQRLQDIVGKGHYRSSNAAIVDAINRLWDHVCEQELDAAYAAAVAENPSYPYESVAERAAARARR
NRRQAVSE

Nucleotide

• Length: 267 bp

>AT212433 NZ_CP107015:123418-123837 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA