Detailed information of TA system TA212431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 817124..817674
• Replicon: plasmid unnamed1
• Accession: NZ_CP081002
• Organism: Sphingobium sp. V4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: K3M67_RS19445
• Protein ID: WP_275490078.1
• Coordinates: 817372..817674 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: K3M67_RS19440
• Protein ID: WP_268169681.1
• Coordinates: 817124..817369 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3M67_RS19415 (K3M67_19415)	813509..813799	+	291	WP_285833059.1	DUF3325 domain-containing protein	-
K3M67_RS19420 (K3M67_19420)	813796..814092	+	297	WP_285833060.1	hypothetical protein	-
K3M67_RS19425 (K3M67_19425)	814266..814883	+	618	WP_285833061.1	energy transducer TonB	-
K3M67_RS19430 (K3M67_19430)	815100..816159	+	1060	Protein_692	IS630 family transposase	-
K3M67_RS19435 (K3M67_19435)	816315..816947	-	633	WP_285833062.1	tyrosine-type recombinase/integrase	-
K3M67_RS19440 (K3M67_19440)	817124..817369	+	246	WP_268169681.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
K3M67_RS19445 (K3M67_19445)	817372..817674	+	303	WP_275490078.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K3M67_RS19450 (K3M67_19450)	817867..818772	+	906	WP_285833063.1	site-specific integrase	-
K3M67_RS19455 (K3M67_19455)	818777..819058	+	282	Protein_697	transposase zinc-binding domain-containing protein	-
K3M67_RS19460 (K3M67_19460)	819332..820348	-	1017	WP_285833733.1	IS110 family transposase	-
K3M67_RS19465 (K3M67_19465)	820445..821365	+	921	Protein_699	transposase	-
K3M67_RS19470 (K3M67_19470)	821791..822228	-	438	WP_285833064.1	GFA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1247975	1247975
	-	flank	IS/Tn	-	-	815920..816159	239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (3-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11359.82 Da
• Isoelectric Point: 5.5124

>T212431 WP_275490078.1 NZ_CP081002:817372-817674 [Sphingobium sp. V4]
VPRFRLTRAAVDDLTAIFLEGIEQFGLPQADTYHEGLSAIFAFLADYPHAARLREEISPPVRVHPYKAHLVIYDVGNEGE
VIILRVRHGREDWISSNYDG

Nucleotide

• Length: 303 bp

>T212431 NZ_CP107015:22425-22868 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8985.05 Da
• Isoelectric Point: 4.7265

>AT212431 WP_268169681.1 NZ_CP081002:817124-817369 [Sphingobium sp. V4]
MATMNISLPDPMKQWVEAQADTGRYSNASDYVRDLIRRDQERADKIAAMQRLADEARASGLSDETMADIRARAISQAGLQ
A

Nucleotide

• Length: 246 bp

>AT212431 NZ_CP107015:22198-22428 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA