Detailed information of TA system TA212430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4977170..4977802
• Replicon: chromosome
• Accession: NZ_CP081000
• Organism: Mycolicibacterium senegalense strain ATCC 35796

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: K3U95_RS23695
• Protein ID: WP_019347713.1
• Coordinates: 4977170..4977619 (-)
• Length: 150 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A378SW37
• Locus tag: K3U95_RS23700
• Protein ID: WP_019347714.1
• Coordinates: 4977623..4977802 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U95_RS23670 (K3U95_23670)	4972225..4973445	+	1221	WP_115150592.1	cytochrome P450	-
K3U95_RS23675 (K3U95_23675)	4973442..4974491	+	1050	WP_036392170.1	dihydrodipicolinate reductase	-
K3U95_RS23680 (K3U95_23680)	4974484..4975542	+	1059	WP_036392168.1	dihydrodipicolinate reductase	-
K3U95_RS23685 (K3U95_23685)	4975539..4975952	+	414	WP_036392166.1	hypothetical protein	-
K3U95_RS23690 (K3U95_23690)	4975945..4977162	+	1218	WP_036392164.1	cytochrome P450	-
K3U95_RS23695 (K3U95_23695)	4977170..4977619	-	450	WP_019347713.1	SRPBCC family protein	Toxin
K3U95_RS23700 (K3U95_23700)	4977623..4977802	-	180	WP_019347714.1	antitoxin	Antitoxin
K3U95_RS23705 (K3U95_23705)	4977861..4980353	-	2493	WP_036392162.1	cation-translocating P-type ATPase	-
K3U95_RS23710 (K3U95_23710)	4980420..4981535	-	1116	WP_036392160.1	MBL fold metallo-hydrolase	-
K3U95_RS23715 (K3U95_23715)	4981537..4982271	-	735	WP_036392158.1	enoyl-CoA hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 150 a.a.
• Molecular weight: 16121.66 Da
• Isoelectric Point: 9.7310

>T212430 WP_019347713.1 NZ_CP081000:c4977619-4977170 [Mycolicibacterium senegalense]
MAKLSVSVEVPLPPDKAWEHASDLSRYDEWLSIHRAWRSKLPETLEKGTVIDSIVEVKGMLNRVKWTLVNYKPPQSLTLN
GDGRGGVKVKLIGKITPATVDGADGAKVSFDVHLGGPALFGPIGMVVAAALKGDIQQSLNKFKELYASS

Nucleotide

• Length: 450 bp

>T212430 NZ_CP107014:c5319955-5319572 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6529.21 Da
• Isoelectric Point: 4.7045

>AT212430 WP_019347714.1 NZ_CP081000:c4977802-4977623 [Mycolicibacterium senegalense]
MGFLDKAKDLLSQNADKVEQAIDKAGDIVDEKTQGKYSQHVDKAQEAAKNALNKEEPQQ

Nucleotide

• Length: 180 bp

>AT212430 NZ_CP107014:c5320197-5319955 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A378SW37