Detailed information of TA system TA212428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 5260690..5261316
• Replicon: chromosome
• Accession: NZ_CP080999
• Organism: Mycobacterium malmoense strain ATCC 29571

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1X0DBA4
• Locus tag: K3U93_RS24395
• Protein ID: WP_071512907.1
• Coordinates: 5260918..5261316 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K3U93_RS24390
• Protein ID: WP_083012693.1
• Coordinates: 5260690..5260917 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U93_RS24375 (K3U93_24375)	5256912..5258381	-	1470	WP_083012691.1	NAD(P)/FAD-dependent oxidoreductase	-
K3U93_RS24380 (K3U93_24380)	5258458..5259114	+	657	WP_071512905.1	TetR/AcrR family transcriptional regulator	-
K3U93_RS24385 (K3U93_24385)	5259423..5260628	+	1206	WP_071512910.1	DNA polymerase IV	-
K3U93_RS24390 (K3U93_24390)	5260690..5260917	+	228	WP_083012693.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K3U93_RS24395 (K3U93_24395)	5260918..5261316	+	399	WP_071512907.1	PIN domain nuclease	Toxin
K3U93_RS24400 (K3U93_24400)	5261369..5262376	-	1008	WP_071512911.1	PHP domain-containing protein	-
K3U93_RS24405 (K3U93_24405)	5262470..5263327	+	858	WP_071512908.1	LLM class F420-dependent oxidoreductase	-
K3U93_RS24410 (K3U93_24410)	5263677..5265143	-	1467	WP_071512909.1	glutamate synthase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-119)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14977.28 Da
• Isoelectric Point: 7.2178

>T212428 WP_071512907.1 NZ_CP080999:5260918-5261316 [Mycobacterium malmoense]
MIVDTSVWIEFLTTSESLASRWLADRIAANSQVIVPEVVMMELLIGTTDESKAIRRRRLLQRFNIEPFAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVTVAHRDRDYEVIRTHCGLQTEPLF

Nucleotide

• Length: 399 bp

>T212428 NZ_CP107014:c4837489-4837205 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8334.16 Da
• Isoelectric Point: 4.0057

>AT212428 WP_083012693.1 NZ_CP080999:5260690-5260917 [Mycobacterium malmoense]
MSRTNIEINDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLDRDEALALQGSGFDFTNDEIESFSDTGIDLADQS

Nucleotide

• Length: 228 bp

>AT212428 NZ_CP107014:c4837721-4837479 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A1X0DBA4