Detailed information of TA system TA212420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3104064..3104661
• Replicon: chromosome
• Accession: NZ_CP080999
• Organism: Mycobacterium malmoense strain ATCC 29571

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K3U93_RS14400
• Protein ID: WP_083012607.1
• Coordinates: 3104064..3104432 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K3U93_RS14405
• Protein ID: WP_071509019.1
• Coordinates: 3104425..3104661 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U93_RS25390 (K3U93_14380)	3099683..3100981	-	1299	WP_176220077.1	PE family protein	-
K3U93_RS14385 (K3U93_14385)	3101480..3102394	+	915	WP_230981261.1	tyrosine-type recombinase/integrase	-
K3U93_RS14390 (K3U93_14390)	3102462..3102580	+	119	Protein_2875	ATPase	-
K3U93_RS14395 (K3U93_14395)	3103655..3103948	+	294	WP_139797206.1	hypothetical protein	-
K3U93_RS14400 (K3U93_14400)	3104064..3104432	-	369	WP_083012607.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K3U93_RS14405 (K3U93_14405)	3104425..3104661	-	237	WP_071509019.1	antitoxin	Antitoxin
K3U93_RS14410 (K3U93_14410)	3105145..3106347	-	1203	WP_071509018.1	hypothetical protein	-
K3U93_RS14415 (K3U93_14415)	3106976..3108007	-	1032	WP_176220078.1	AAA family ATPase	-
K3U93_RS14420 (K3U93_14420)	3108233..3109513	-	1281	WP_071509016.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3099683..3122679	22996

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 12736.68 Da
• Isoelectric Point: 6.0706

>T212420 WP_083012607.1 NZ_CP080999:c3104432-3104064 [Mycobacterium malmoense]
MPEPRRGELWLVALGAARAGGPGKHHPAVVVSVDEILTGLDDELVVVVPVSSSRSGSPLRPPVMPAEGVDTQSVAVCRDI
RAVARSRLVERLGTVAPDTMHGIEHALGLILGVDTGLSPPSW

Nucleotide

• Length: 369 bp

>T212420 NZ_CP107014:319695-320063 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8404.25 Da
• Isoelectric Point: 4.5742

>AT212420 WP_071509019.1 NZ_CP080999:c3104661-3104425 [Mycobacterium malmoense]
MSTSTTTIRVSVRTRDRLVAQARQRGISLAGLVTELASRAEQEAVFRAERDATLADAGIAGVREEDRDWDDTAGDGIA

Nucleotide

• Length: 237 bp

>AT212420 NZ_CP107014:319477-319698 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA