Detailed information of TA system TA212412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 829017..829582
• Replicon: chromosome
• Accession: NZ_CP080999
• Organism: Mycobacterium malmoense strain ATCC 29571

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: K3U93_RS03860
• Protein ID: WP_277622396.1
• Coordinates: 829017..829334 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: K3U93_RS03865
• Protein ID: WP_277622374.1
• Coordinates: 829349..829582 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U93_RS03835 (K3U93_03835)	824384..825631	-	1248	WP_083009269.1	FAD-dependent monooxygenase	-
K3U93_RS03840 (K3U93_03840)	825707..826243	+	537	WP_083009272.1	TetR/AcrR family transcriptional regulator	-
K3U93_RS03845 (K3U93_03845)	826273..827262	+	990	WP_083009274.1	LLM class F420-dependent oxidoreductase	-
K3U93_RS03850 (K3U93_03850)	827346..827741	-	396	WP_230981573.1	hypothetical protein	-
K3U93_RS03855 (K3U93_03855)	828067..828969	+	903	WP_083009277.1	DMT family transporter	-
K3U93_RS03860 (K3U93_03860)	829017..829334	-	318	WP_277622396.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K3U93_RS03865 (K3U93_03865)	829349..829582	-	234	WP_277622374.1	antitoxin	Antitoxin
K3U93_RS03870 (K3U93_03870)	830001..830267	-	267	WP_083009283.1	hypothetical protein	-
K3U93_RS03875 (K3U93_03875)	830340..830606	+	267	WP_139796754.1	hypothetical protein	-
K3U93_RS03880 (K3U93_03880)	830831..831049	-	219	WP_083009286.1	antitoxin	-
K3U93_RS03885 (K3U93_03885)	831220..831465	+	246	WP_139796755.1	hypothetical protein	-
K3U93_RS03890 (K3U93_03890)	831488..831847	+	360	WP_230981574.1	type II toxin-antitoxin system VapC family toxin	-
K3U93_RS03895 (K3U93_03895)	831780..832217	-	438	WP_254893541.1	helix-turn-helix transcriptional regulator	-
K3U93_RS03900 (K3U93_03900)	832869..834047	+	1179	WP_083009288.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	827771..842415	14644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11253.97 Da
• Isoelectric Point: 11.6144

>T212412 WP_277622396.1 NZ_CP080999:c829334-829017 [Mycobacterium malmoense]
IFIVSLDPTQGHEQRGTRPVLIVSPTAFNRLTQTPVVLPITRGGNFARTAGFAVSLTDAGTRTTGVIRCDQPRAIDIGSR
NGRKVERVPAAILDEVLAKLATLFS

Nucleotide

• Length: 318 bp

>T212412 NZ_CP107010:3379866-3380084 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACAATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8109.19 Da
• Isoelectric Point: 3.9500

>AT212412 WP_277622374.1 NZ_CP080999:c829582-829349 [Mycobacterium malmoense]
VGGSVMLAVPPAVLDMLRLHAGSAVGLSVDAGHLIVEPQTRPRYTLDELLSQCDASADLTDEDRVWLDGEPVGGELL

Nucleotide

• Length: 234 bp

>AT212412 NZ_CP107010:3379464-3379838 [Salmonella enterica subsp. enterica serovar Paratyphi B]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAAC
ATATTGCAACTTTCGCTCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA