Detailed information of TA system TA212402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2483030..2483565
• Replicon: chromosome
• Accession: NZ_CP080997
• Organism: Mycolicibacter heraklionensis strain JCM 30995

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K3U94_RS11685
• Protein ID: WP_220696578.1
• Coordinates: 2483233..2483565 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A0I9TM53
• Locus tag: K3U94_RS11680
• Protein ID: WP_047317851.1
• Coordinates: 2483030..2483236 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U94_RS11650 (K3U94_11650)	2478570..2478998	+	429	WP_082133944.1	cellulose-binding domain-containing protein	-
K3U94_RS11655 (K3U94_11655)	2479007..2479573	-	567	WP_220696574.1	dihydrofolate reductase family protein	-
K3U94_RS11660 (K3U94_11660)	2479624..2479770	+	147	WP_220696575.1	hypothetical protein	-
K3U94_RS11665 (K3U94_11665)	2480211..2481413	+	1203	WP_220696576.1	hypothetical protein	-
K3U94_RS11670 (K3U94_11670)	2481432..2481983	-	552	WP_220696577.1	YaeQ family protein	-
K3U94_RS11675 (K3U94_11675)	2482104..2482811	-	708	WP_131721466.1	hypothetical protein	-
K3U94_RS11680 (K3U94_11680)	2483030..2483236	+	207	WP_047317851.1	DUF3018 family protein	Antitoxin
K3U94_RS11685 (K3U94_11685)	2483233..2483565	+	333	WP_220696578.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K3U94_RS11690 (K3U94_11690)	2484011..2485135	+	1125	WP_074322231.1	tyrosine-type recombinase/integrase	-
K3U94_RS11695 (K3U94_11695)	2485128..2485490	+	363	WP_074322232.1	helix-turn-helix transcriptional regulator	-
K3U94_RS11700 (K3U94_11700)	2485468..2486925	+	1458	WP_074322233.1	Fis family transcriptional regulator	-
K3U94_RS11705 (K3U94_11705)	2486992..2487585	-	594	WP_074322240.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11862.68 Da
• Isoelectric Point: 5.6845

>T212402 WP_220696578.1 NZ_CP080997:2483233-2483565 [Mycolicibacter heraklionensis]
VNRGEIWTVAGGVYANKPRPAVIVEDDLFDATSSVTVAPMSSTLLDAPLMRIRIAGGDGLLSGLDHDSDVMIDKLTTVKR
SNVHTRLGRLTAEQLVEVERAMMAFLGLAR

Nucleotide

• Length: 333 bp

>T212402 NZ_CP107007:c985179-985042 [Halomonas sp. GD1P12]
ATGCTTTTGCTTGAAGATTTTGAAGCGCTAAAGGAAACCGCCTATCTACTGCGTTCACCCAATAACGCACGCCGTTTGAT
GGACGCAATGGACGAATTGGAAAAAGGCGGCGGCCAGGAAAAGGAGCTACTTGAGTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 8047.96 Da
• Isoelectric Point: 4.8096

>AT212402 WP_047317851.1 NZ_CP080997:2483030-2483236 [Mycolicibacter heraklionensis]
MAVRERVSEYRRRMRERGLRPLQVWVPDVRTESFVAEAHRQASMVARADVSNDDQDFIEAISTPWDEE

Nucleotide

• Length: 207 bp

>AT212402 NZ_CP107007:c985045-984791 [Halomonas sp. GD1P12]
GTGAAGCTCGTCTTTGCCGAGAATGCTTGGGAAGATTACTTGTACTGGCAAAAAACCGACAAGAAAATTCCTGGTCGCAT
CAACAAGCTGATCAAGGAGATTGAGCGACAGCCGTTTGAGGGCGTTGGAAAACCCGAACCGCTCAAGCATGGCTTTGCGG
GCTACTGGTCCCGGCGAATCAACGATGAGCATCGGATCGTTTACAAGGTGGCGGACGATGCGCTGCTGATTGCGCAGCTT
CGATATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0I9TM53