Detailed information of TA system TA212401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 2462756..2463720
• Replicon: chromosome
• Accession: NZ_CP080997
• Organism: Mycolicibacter heraklionensis strain JCM 30995

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: K3U94_RS11570
• Protein ID: WP_220696770.1
• Coordinates: 2463142..2463720 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A0I9TQB1
• Locus tag: K3U94_RS11565
• Protein ID: WP_047317834.1
• Coordinates: 2462756..2463145 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U94_RS11540 (K3U94_11540)	2458134..2458949	+	816	WP_220696560.1	SAM-dependent methyltransferase	-
K3U94_RS11545 (K3U94_11545)	2458925..2460541	-	1617	WP_220696561.1	FAD-binding protein	-
K3U94_RS11550 (K3U94_11550)	2460730..2461575	+	846	WP_220696562.1	lytic transglycosylase domain-containing protein	-
K3U94_RS11555 (K3U94_11555)	2461580..2461945	-	366	WP_220696563.1	RNA-binding S4 domain-containing protein	-
K3U94_RS11560 (K3U94_11560)	2461958..2462560	-	603	WP_220696769.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
K3U94_RS11565 (K3U94_11565)	2462756..2463145	+	390	WP_047317834.1	hypothetical protein	Antitoxin
K3U94_RS11570 (K3U94_11570)	2463142..2463720	+	579	WP_220696770.1	zeta toxin family protein	Toxin
K3U94_RS11575 (K3U94_11575)	2463717..2464730	-	1014	WP_220696564.1	NADP-dependent oxidoreductase	-
K3U94_RS11580 (K3U94_11580)	2464804..2465454	-	651	WP_220696565.1	maleylpyruvate isomerase family mycothiol-dependent enzyme	-
K3U94_RS11585 (K3U94_11585)	2465461..2466024	-	564	WP_230987550.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
K3U94_RS11590 (K3U94_11590)	2466080..2467117	+	1038	WP_220696566.1	TIGR03617 family F420-dependent LLM class oxidoreductase	-
K3U94_RS11595 (K3U94_11595)	2467128..2468021	+	894	WP_220696567.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-135)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 20725.93 Da
• Isoelectric Point: 10.1524

>T212401 WP_220696770.1 NZ_CP080997:2463142-2463720 [Mycolicibacter heraklionensis]
MRRLDLVVGPNGAGKSTFVALTLAPLLPGALFVNADEIAKTRWPDEVAAHAYDAARLAAKTRMKLIEAGRSFIAETVFSH
GSKIDLIHGAHAHGYTVVLHAVLIPEALAVQRVRYRVAAGGHAVPEEKIRERYHRVWPLVAEAIGIVDVATVYDNSRVTG
PKIVAQFAGGAQIGDPSWPSWAPPALVTRRHP

Nucleotide

• Length: 579 bp

>T212401 NZ_CP107007:c985045-984791 [Halomonas sp. GD1P12]
GTGAAGCTCGTCTTTGCCGAGAATGCTTGGGAAGATTACTTGTACTGGCAAAAAACCGACAAGAAAATTCCTGGTCGCAT
CAACAAGCTGATCAAGGAGATTGAGCGACAGCCGTTTGAGGGCGTTGGAAAACCCGAACCGCTCAAGCATGGCTTTGCGG
GCTACTGGTCCCGGCGAATCAACGATGAGCATCGGATCGTTTACAAGGTGGCGGACGATGCGCTGCTGATTGCGCAGCTT
CGATATCATTATTGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13760.17 Da
• Isoelectric Point: 4.7939

>AT212401 WP_047317834.1 NZ_CP080997:2462756-2463145 [Mycolicibacter heraklionensis]
MAEVADRVTRFAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSAQQSAARRRVEAALSGHLETGALTAEEGVVFNAEI
SAAIEEHLAHTHYGDILAERGVTTVALDDDGRIVEYRPDGTSVIVEPTP

Nucleotide

• Length: 390 bp

>AT212401 NZ_CP107007:c985179-985042 [Halomonas sp. GD1P12]
ATGCTTTTGCTTGAAGATTTTGAAGCGCTAAAGGAAACCGCCTATCTACTGCGTTCACCCAATAACGCACGCCGTTTGAT
GGACGCAATGGACGAATTGGAAAAAGGCGGCGGCCAGGAAAAGGAGCTACTTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0I9TQB1