Detailed information of TA system TA212398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1432279..1432769
• Replicon: chromosome
• Accession: NZ_CP080997
• Organism: Mycolicibacter heraklionensis strain JCM 30995

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K3U94_RS06835
• Protein ID: WP_230987460.1
• Coordinates: 1432569..1432769 (-)
• Length: 67 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A0I9TH85
• Locus tag: K3U94_RS06830
• Protein ID: WP_047320114.1
• Coordinates: 1432279..1432515 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U94_RS06805 (K3U94_06805)	1428430..1429320	+	891	WP_220696006.1	helix-turn-helix domain-containing protein	-
K3U94_RS06810 (K3U94_06810)	1429397..1430284	+	888	WP_047320158.1	transcriptional regulator CynR	-
K3U94_RS06815 (K3U94_06815)	1430383..1430886	+	504	WP_047320116.1	nucleoside deaminase	-
K3U94_RS06820 (K3U94_06820)	1430941..1431378	+	438	WP_047320157.1	MarR family transcriptional regulator	-
K3U94_RS06825 (K3U94_06825)	1431445..1432263	+	819	WP_047320115.1	uracil-DNA glycosylase	-
K3U94_RS06830 (K3U94_06830)	1432279..1432515	-	237	WP_047320114.1	hypothetical protein	Antitoxin
K3U94_RS06835 (K3U94_06835)	1432569..1432769	-	201	WP_230987460.1	BrnT family toxin	Toxin
K3U94_RS06840 (K3U94_06840)	1432866..1433897	+	1032	WP_220696703.1	LLM class flavin-dependent oxidoreductase	-
K3U94_RS06845 (K3U94_06845)	1433939..1434319	-	381	WP_220696007.1	type II toxin-antitoxin system VapC family toxin	-
K3U94_RS06850 (K3U94_06850)	1434316..1434564	-	249	WP_220696008.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-64)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 67 a.a.
• Molecular weight: 7474.46 Da
• Isoelectric Point: 5.7298

>T212398 WP_230987460.1 NZ_CP080997:c1432769-1432569 [Mycolicibacter heraklionensis]
VLAFEFDPAKSAANLTKHGIDFDVAQSIWSDPRRIEVPARTTDEPRWMAIGQIDGRCWSAVLTYPR

Nucleotide

• Length: 201 bp

>T212398 NZ_CP107005:c4119378-4119028 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8888.98 Da
• Isoelectric Point: 6.2216

>AT212398 WP_047320114.1 NZ_CP080997:c1432515-1432279 [Mycolicibacter heraklionensis]
MKADEFDERFDNGQDMAAALDTARARRPGEQHRRVNVDFPVWMIAELDREATRLGVTRQSLIKVWIAEKLDHGGHTAA

Nucleotide

• Length: 237 bp

>AT212398 NZ_CP107005:c4119623-4119372 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0I9TH85