Detailed information of TA system TA212396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 757321..757991
• Replicon: chromosome
• Accession: NZ_CP080997
• Organism: Mycolicibacter heraklionensis strain JCM 30995

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3U94_RS03590
• Protein ID: WP_220695603.1
• Coordinates: 757321..757719 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K3U94_RS03595
• Protein ID: WP_220695604.1
• Coordinates: 757719..757991 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U94_RS03570 (K3U94_03570)	752757..754175	-	1419	WP_220695600.1	SDR family oxidoreductase	-
K3U94_RS03575 (K3U94_03575)	754246..755886	+	1641	WP_220695601.1	peptide chain release factor 3	-
K3U94_RS03580 (K3U94_03580)	755895..756776	-	882	WP_047320927.1	NYN domain-containing protein	-
K3U94_RS03585 (K3U94_03585)	756972..757280	-	309	WP_220695602.1	hypothetical protein	-
K3U94_RS03590 (K3U94_03590)	757321..757719	-	399	WP_220695603.1	type II toxin-antitoxin system VapC family toxin	Toxin
K3U94_RS03595 (K3U94_03595)	757719..757991	-	273	WP_220695604.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
K3U94_RS03600 (K3U94_03600)	758032..759771	-	1740	WP_220695605.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-100)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-44)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14150.31 Da
• Isoelectric Point: 5.7185

>T212396 WP_220695603.1 NZ_CP080997:c757719-757321 [Mycolicibacter heraklionensis]
MLYLDTSALVKLVRREPESDALVDWLAAQQPTPWVSSTLIEAELPRAIRRAEPSLLVDVPATLARVSRYEVNEVVRAAAA
ALPDPTLRSLDAIHLATGHAVFGARLTAFVAYDGRLLAAAEAIGLPTVSPGR

Nucleotide

• Length: 399 bp

>T212396 NZ_CP107005:3759975-3760253 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9890.21 Da
• Isoelectric Point: 6.2260

>AT212396 WP_220695604.1 NZ_CP080997:c757991-757719 [Mycolicibacter heraklionensis]
MSEVPVRELNQNTAGVLARVKRGEHLEITERGTVVARLVPAQDSPLAEMIKAGQLHPATVSGPVPRPTGPVRTDHEAGDL
LREMRDDERY

Nucleotide

• Length: 273 bp

>AT212396 NZ_CP107005:3759712-3759972 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA