Detailed information of TA system TA212395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 719161..719798
• Replicon: chromosome
• Accession: NZ_CP080997
• Organism: Mycolicibacter heraklionensis strain JCM 30995

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3U94_RS03420
• Protein ID: WP_220695576.1
• Coordinates: 719400..719798 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0I9TAF8
• Locus tag: K3U94_RS03415
• Protein ID: WP_047320910.1
• Coordinates: 719161..719403 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U94_RS03380 (K3U94_03380)	714186..714548	-	363	WP_047320906.1	DoxX family protein	-
K3U94_RS03385 (K3U94_03385)	714658..715440	+	783	WP_220695571.1	enoyl-CoA hydratase/isomerase family protein	-
K3U94_RS03390 (K3U94_03390)	715446..715853	+	408	WP_220695572.1	hypothetical protein	-
K3U94_RS03395 (K3U94_03395)	715850..716296	-	447	WP_220695573.1	hypothetical protein	-
K3U94_RS03400 (K3U94_03400)	716371..717156	-	786	WP_220695574.1	S1 family peptidase	-
K3U94_RS03405 (K3U94_03405)	717327..717647	+	321	WP_230987380.1	hypothetical protein	-
K3U94_RS03410 (K3U94_03410)	717668..719095	+	1428	WP_220695575.1	YdiU family protein	-
K3U94_RS03415 (K3U94_03415)	719161..719403	+	243	WP_047320910.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K3U94_RS03420 (K3U94_03420)	719400..719798	+	399	WP_220695576.1	type II toxin-antitoxin system VapC family toxin	Toxin
K3U94_RS03425 (K3U94_03425)	719815..722148	-	2334	WP_220696652.1	MMPL family transporter	-
K3U94_RS03430 (K3U94_03430)	722333..723349	+	1017	WP_220695577.1	NAD(P)/FAD-dependent oxidoreductase	-
K3U94_RS03435 (K3U94_03435)	723234..724385	-	1152	WP_220696653.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-121)

Antitoxin

• Antitoxin VapB-like (1-80)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14352.09 Da
• Isoelectric Point: 4.3124

>T212395 WP_220695576.1 NZ_CP080997:719400-719798 [Mycolicibacter heraklionensis]
MIVDTSAIIAILRDEDDAPRYARAIAGAQSRRLSAAAYLECGIVLDSQRDPIISRALDELLDDAGFVIEPVTERQARLAR
QAYADFGKGSSHPAGLNFGDCLSYALATDLREPLLWKGDDFGYTGIRSACDE

Nucleotide

• Length: 399 bp

>T212395 NZ_CP107005:c3753913-3753515 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8651.94 Da
• Isoelectric Point: 10.0829

>AT212395 WP_047320910.1 NZ_CP080997:719161-719403 [Mycolicibacter heraklionensis]
MALNIKDPAVHEAVKEIARITGESQAQAVATAVRDRLVHVQADAAAARLLAIGRRTAARMSPETKRLDHGTLLYDQHGMP

Nucleotide

• Length: 243 bp

>AT212395 NZ_CP107005:c3754209-3753916 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0I9TAF8