Detailed information of TA system TA212394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 101098..101645
• Replicon: chromosome
• Accession: NZ_CP080997
• Organism: Mycolicibacter heraklionensis strain JCM 30995

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K3U94_RS00515
• Protein ID: WP_220695260.1
• Coordinates: 101334..101645 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K3U94_RS00510
• Protein ID: WP_275564537.1
• Coordinates: 101098..101334 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U94_RS00475 (K3U94_00475)	96382..97320	+	939	WP_220695258.1	class I SAM-dependent methyltransferase	-
K3U94_RS00480 (K3U94_00480)	97404..98264	+	861	WP_047320341.1	SDR family oxidoreductase	-
K3U94_RS00485 (K3U94_00485)	98299..99114	-	816	WP_220695259.1	tyrosine-protein phosphatase	-
K3U94_RS00490 (K3U94_00490)	99218..99361	-	144	WP_095173665.1	hypothetical protein	-
K3U94_RS00495 (K3U94_00495)	99495..100142	+	648	WP_047320342.1	TetR/AcrR family transcriptional regulator	-
K3U94_RS00500 (K3U94_00500)	100170..100571	-	402	WP_052956954.1	type II toxin-antitoxin system VapC family toxin	-
K3U94_RS00505 (K3U94_00505)	100661..100816	-	156	Protein_97	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K3U94_RS00510 (K3U94_00510)	101098..101334	+	237	WP_275564537.1	YlcI/YnfO family protein	Antitoxin
K3U94_RS00515 (K3U94_00515)	101334..101645	+	312	WP_220695260.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K3U94_RS00520 (K3U94_00520)	102139..102435	-	297	WP_220694754.1	metalloregulator ArsR/SmtB family transcription factor	-
K3U94_RS00525 (K3U94_00525)	102818..103735	+	918	WP_220695261.1	sulfurtransferase	-
K3U94_RS00530 (K3U94_00530)	103746..104330	+	585	WP_220695262.1	hypothetical protein	-
K3U94_RS00535 (K3U94_00535)	104484..105647	-	1164	WP_230987328.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 10990.72 Da
• Isoelectric Point: 5.5815

>T212394 WP_220695260.1 NZ_CP080997:101334-101645 [Mycolicibacter heraklionensis]
MRPIHIALLDKSRPVLILTRELVRPHLTNVTVAPITSTVRGLSTEVPVGAANGLNQPSVVSCDNIQTIPVVDLGRQIGYL
LASQESALADAIGNAFDLDWVVP

Nucleotide

• Length: 312 bp

>T212394 NZ_CP107005:3743321-3743662 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8578.76 Da
• Isoelectric Point: 7.0149

>AT212394 WP_275564537.1 NZ_CP080997:101098-101334 [Mycolicibacter heraklionensis]
VSTQIAVRVPDDLIAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAVILAATASAPTEMDALAGRSARTILDID

Nucleotide

• Length: 237 bp

>AT212394 NZ_CP107005:3742983-3743300 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA