Detailed information of TA system TA212393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 64975..65606
• Replicon: chromosome
• Accession: NZ_CP080997
• Organism: Mycolicibacter heraklionensis strain JCM 30995

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K3U94_RS00305
• Protein ID: WP_267878368.1
• Coordinates: 64975..65385 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0I9TD89
• Locus tag: K3U94_RS00310
• Protein ID: WP_047320315.1
• Coordinates: 65388..65606 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3U94_RS00285 (K3U94_00285)	61003..62493	-	1491	WP_220695234.1	type VII secretion protein EccB	-
K3U94_RS00290 (K3U94_00290)	62643..63551	+	909	WP_230987324.1	hypothetical protein	-
K3U94_RS23410	63660..64271	+	612	WP_230987325.1	DUF4226 domain-containing protein	-
K3U94_RS00300 (K3U94_00300)	64308..64937	+	630	WP_220695235.1	sensor domain-containing protein	-
K3U94_RS00305 (K3U94_00305)	64975..65385	-	411	WP_267878368.1	type II toxin-antitoxin system VapC family toxin	Toxin
K3U94_RS00310 (K3U94_00310)	65388..65606	-	219	WP_047320315.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
K3U94_RS00315 (K3U94_00315)	65679..66638	-	960	WP_220695236.1	hypothetical protein	-
K3U94_RS23415	66635..67732	-	1098	WP_230987326.1	DUF5631 domain-containing protein	-
K3U94_RS00325 (K3U94_00325)	69141..69515	-	375	WP_220695238.1	hypothetical protein	-
K3U94_RS00330 (K3U94_00330)	69865..70347	-	483	WP_230987671.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (12-132)

Antitoxin

• Post-segregation antitoxin CcdA (3-45)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14723.91 Da
• Isoelectric Point: 6.7282

>T212393 WP_267878368.1 NZ_CP080997:c65385-64975 [Mycolicibacter heraklionensis]
ISSSSSPAEQVVIDASAMVDLLARTRDRFTAVRARLANTVMNAPTHFDAEVVSALGRMHRAGVLTVAQVETALDELRRAP
VIKHELSPLLAGAWARRDILRLTDALYVELADTANLVLLTTDQRLARAWPAAEAIG

Nucleotide

• Length: 411 bp

>T212393 NZ_CP107005:3561455-3561673 [Escherichia coli]
ATGTCCGAAAAATTTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7845.57 Da
• Isoelectric Point: 4.2537

>AT212393 WP_047320315.1 NZ_CP080997:c65606-65388 [Mycolicibacter heraklionensis]
MARLNVYVPDDLAERARARGLNVSALTQAAISAELENSGTRTWLDGLEDRSTTARHDDVLDALDTARDEFGA

Nucleotide

• Length: 219 bp

>AT212393 NZ_CP107005:3561055-3561429 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0I9TD89