Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4714979..4715555 Replicon chromosome
Accession NZ_CP080960
Organism Salmonella enterica subsp. enterica serovar Typhi strain ISP2825

Toxin (Protein)


Gene name shpA Uniprot ID M7RG88
Locus tag K3U68_RS23135 Protein ID WP_001131963.1
Coordinates 4714979..4715266 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID B5R9R5
Locus tag K3U68_RS23140 Protein ID WP_000063142.1
Coordinates 4715253..4715555 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3U68_RS23125 (K3U68_23125) 4710105..4713614 - 3510 WP_001043493.1 type I restriction-modification system endonuclease -
K3U68_RS23130 (K3U68_23130) 4713812..4714726 + 915 WP_000290515.1 restriction endonuclease -
K3U68_RS23135 (K3U68_23135) 4714979..4715266 + 288 WP_001131963.1 BrnT family toxin Toxin
K3U68_RS23140 (K3U68_23140) 4715253..4715555 + 303 WP_000063142.1 BrnA antitoxin family protein Antitoxin
K3U68_RS23145 (K3U68_23145) 4715630..4716586 - 957 WP_000187846.1 GTPase -
K3U68_RS23150 (K3U68_23150) 4716597..4716800 - 204 WP_000460616.1 YbdD/YjiX family protein -
K3U68_RS23155 (K3U68_23155) 4716895..4719045 - 2151 WP_000379928.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11294.80 Da        Isoelectric Point: 9.5894

>T212392 WP_001131963.1 NZ_CP080960:4714979-4715266 [Salmonella enterica subsp. enterica serovar Typhi]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T212392 NZ_CP107005:2671044-2671151 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.96 Da        Isoelectric Point: 10.1293

>AT212392 WP_000063142.1 NZ_CP080960:4715253-4715555 [Salmonella enterica subsp. enterica serovar Typhi]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Download         Length: 303 bp

>AT212392 NZ_CP107005:c2670997-2670931 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VD7

Antitoxin

Source ID Structure
PDB 7VD7
AlphaFold DB A0A4D6PB01

References