Detailed information of TA system TA212373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4658028..4658656
• Replicon: chromosome
• Accession: NZ_CP080958
• Organism: Citrobacter amalonaticus strain C3H

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7L6Z8W6
• Locus tag: K1713_RS22365
• Protein ID: WP_044257173.1
• Coordinates: 4658028..4658414 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K1713_RS22370
• Protein ID: WP_161215612.1
• Coordinates: 4658414..4658656 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1713_RS22345 (K1713_22345)	4653381..4654718	+	1338	WP_086512772.1	VWA domain-containing protein	-
K1713_RS22350 (K1713_22350)	4654736..4656232	+	1497	WP_044257176.1	HAMP domain-containing sensor histidine kinase	-
K1713_RS22355 (K1713_22355)	4656237..4656923	+	687	WP_044257174.1	response regulator transcription factor	-
K1713_RS22360 (K1713_22360)	4657068..4657997	+	930	WP_042998866.1	formate dehydrogenase accessory protein FdhE	-
K1713_RS22365 (K1713_22365)	4658028..4658414	-	387	WP_044257173.1	type II toxin-antitoxin system VapC family toxin	Toxin
K1713_RS22370 (K1713_22370)	4658414..4658656	-	243	WP_161215612.1	CopG family transcriptional regulator	Antitoxin
K1713_RS22375 (K1713_22375)	4658864..4659790	+	927	WP_044257169.1	alpha/beta hydrolase	-
K1713_RS22380 (K1713_22380)	4659806..4660747	-	942	WP_042998862.1	fatty acid biosynthesis protein FabY	-
K1713_RS22385 (K1713_22385)	4660792..4661229	-	438	WP_044265155.1	D-aminoacyl-tRNA deacylase	-
K1713_RS22390 (K1713_22390)	4661226..4662098	-	873	WP_044257165.1	virulence factor BrkB family protein	-
K1713_RS22395 (K1713_22395)	4662092..4662691	-	600	WP_044265152.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14403.70 Da
• Isoelectric Point: 9.4114

>T212373 WP_044257173.1 NZ_CP080958:c4658414-4658028 [Citrobacter amalonaticus]
MQKGPVLFDTNILIDLFSGRQEAQQVLEAYPPQNAISLITWMEVMVGAKKYHQEYRTRVALSAFNIIGVSLEIAERSVNL
RQEYGMKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQAGR

Nucleotide

• Length: 387 bp

>T212373 NZ_CP107004:4032571-4032828 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9335.53 Da
• Isoelectric Point: 4.2075

>AT212373 WP_161215612.1 NZ_CP080958:c4658656-4658414 [Citrobacter amalonaticus]
IMAGMDMGRILLDLSDEVIKRLDDLKQQRNLPRAELLREAVEQYLERQGQAETTISNALGLWQGCEEDGVEYQRTLREEW

Nucleotide

• Length: 243 bp

>AT212373 NZ_CP107004:4032840-4033385 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: A0A7L6Z8W6