Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4015245..4015899 Replicon chromosome
Accession NZ_CP080958
Organism Citrobacter amalonaticus strain C3H

Toxin (Protein)


Gene name cptA Uniprot ID A0A3Q8D714
Locus tag K1713_RS19365 Protein ID WP_044254016.1
Coordinates 4015245..4015652 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0F6TWP9
Locus tag K1713_RS19370 Protein ID WP_042322123.1
Coordinates 4015633..4015899 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K1713_RS19345 (K1713_19345) 4011150..4012883 - 1734 WP_042999369.1 single-stranded-DNA-specific exonuclease RecJ -
K1713_RS19350 (K1713_19350) 4012889..4013602 - 714 WP_042999368.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K1713_RS19355 (K1713_19355) 4013626..4014522 - 897 WP_042999367.1 site-specific tyrosine recombinase XerD -
K1713_RS19360 (K1713_19360) 4014623..4015144 + 522 WP_044254018.1 flavodoxin FldB -
K1713_RS19365 (K1713_19365) 4015245..4015652 - 408 WP_044254016.1 protein YgfX Toxin
K1713_RS19370 (K1713_19370) 4015633..4015899 - 267 WP_042322123.1 FAD assembly factor SdhE Antitoxin
K1713_RS19375 (K1713_19375) 4016156..4017136 + 981 WP_044254013.1 tRNA-modifying protein YgfZ -
K1713_RS19380 (K1713_19380) 4017217..4017876 - 660 WP_042999363.1 hemolysin III family protein -
K1713_RS19385 (K1713_19385) 4018039..4018350 - 312 WP_044254010.1 N(4)-acetylcytidine aminohydrolase -
K1713_RS19390 (K1713_19390) 4018403..4019131 + 729 WP_044254008.1 MurR/RpiR family transcriptional regulator -
K1713_RS19395 (K1713_19395) 4019251..4020684 + 1434 WP_044254006.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15863.86 Da        Isoelectric Point: 11.6030

>T212371 WP_044254016.1 NZ_CP080958:c4015652-4015245 [Citrobacter amalonaticus]
VVLWQSDLRVSWRAQWLSLLLHGLVAAAILLMPWPLSYTPLWLILLSLVVFDCVRSQRRINARQGEVKLLMDGRLRWQGQ
DWLIVSAPWMIKTGMMLRLRSETGKRQHLWLAADSMDKAEWRDLRRLMMQPSTQG

Download         Length: 408 bp

>T212371 NZ_CP107004:c3753913-3753515 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10544.06 Da        Isoelectric Point: 5.1496

>AT212371 WP_042322123.1 NZ_CP080958:c4015899-4015633 [Citrobacter amalonaticus]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RDRGPVAI

Download         Length: 267 bp

>AT212371 NZ_CP107004:c3754209-3753916 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D714

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6TWP9

References