Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1330218..1330838 Replicon chromosome
Accession NZ_CP080958
Organism Citrobacter amalonaticus strain C3H

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag K1713_RS06385 Protein ID WP_001280991.1
Coordinates 1330218..1330436 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag K1713_RS06390 Protein ID WP_042324455.1
Coordinates 1330464..1330838 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K1713_RS06355 (K1713_06355) 1325871..1326467 + 597 WP_200015811.1 hypothetical protein -
K1713_RS06360 (K1713_06360) 1326550..1326903 + 354 WP_200015810.1 DUF1428 family protein -
K1713_RS06365 (K1713_06365) 1326943..1328493 - 1551 WP_090048827.1 EAL domain-containing protein -
K1713_RS06370 (K1713_06370) 1328716..1328856 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
K1713_RS06375 (K1713_06375) 1328906..1329376 - 471 WP_200015809.1 YlaC family protein -
K1713_RS06380 (K1713_06380) 1329490..1330041 - 552 WP_200015808.1 maltose O-acetyltransferase -
K1713_RS06385 (K1713_06385) 1330218..1330436 - 219 WP_001280991.1 HHA domain-containing protein Toxin
K1713_RS06390 (K1713_06390) 1330464..1330838 - 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
K1713_RS06395 (K1713_06395) 1331350..1334499 - 3150 WP_043001557.1 efflux RND transporter permease AcrB -
K1713_RS06400 (K1713_06400) 1334522..1335715 - 1194 WP_043001556.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T212361 WP_001280991.1 NZ_CP080958:c1330436-1330218 [Citrobacter amalonaticus]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T212361 NZ_CP107004:1800483-1800737 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT212361 WP_042324455.1 NZ_CP080958:c1330838-1330464 [Citrobacter amalonaticus]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT212361 NZ_CP107004:1800235-1800486 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References