Detailed information of TA system TA212359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-RHH
• Location: 2426892..2427526
• Replicon: chromosome
• Accession: NZ_CP080953
• Organism: Hydrogenibacillus sp. N12

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A132NF83
• Locus tag: K2M58_RS11405
• Protein ID: WP_066199199.1
• Coordinates: 2426892..2427242 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K2M58_RS11410
• Protein ID: WP_252393476.1
• Coordinates: 2427245..2427526 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2M58_RS11385 (K2M58_11385)	2422449..2422949	-	501	WP_066199206.1	ribosomal protein S18-alanine N-acetyltransferase	-
K2M58_RS11390 (K2M58_11390)	2422946..2423824	-	879	WP_220677634.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-
K2M58_RS11395 (K2M58_11395)	2423802..2424230	-	429	WP_220678914.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
K2M58_RS11400 (K2M58_11400)	2424434..2426752	-	2319	WP_220677635.1	methyl-accepting chemotaxis protein	-
K2M58_RS11405 (K2M58_11405)	2426892..2427242	-	351	WP_066199199.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K2M58_RS11410 (K2M58_11410)	2427245..2427526	-	282	WP_252393476.1	ribbon-helix-helix protein, CopG family	Antitoxin
K2M58_RS11415 (K2M58_11415)	2427723..2428898	-	1176	WP_220677636.1	alanine racemase	-
K2M58_RS11420 (K2M58_11420)	2428989..2430008	-	1020	WP_220677637.1	outer membrane lipoprotein carrier protein LolA	-
K2M58_RS11425 (K2M58_11425)	2430036..2430452	-	417	WP_220677638.1	holo-ACP synthase	-
K2M58_RS11430 (K2M58_11430)	2430533..2430880	+	348	WP_220677639.1	hypothetical protein	-
K2M58_RS11435 (K2M58_11435)	2430974..2431837	-	864	WP_066199183.1	2-oxoacid:ferredoxin oxidoreductase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12805.86 Da
• Isoelectric Point: 6.7259

>T212359 WP_066199199.1 NZ_CP080953:c2427242-2426892 [Hydrogenibacillus sp. N12]
MIVRRGDVFFANLSPVVGSEQGGMRPVLVIQNDIGNRFSPTVIVAAITSQIQKAKLPTHVEISGKAHGLDRDSVILLEQI
RTIDKQRLTDKITHLDDEVMSRVNEALMISLGLVDF

Nucleotide

• Length: 351 bp

>T212359 NZ_CP107004:c1123275-1122202 [Escherichia coli]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10597.23 Da
• Isoelectric Point: 6.4788

>AT212359 WP_252393476.1 NZ_CP080953:c2427526-2427245 [Hydrogenibacillus sp. N12]
MGKEGAVKRIVVSVPQDLLAEVDGLAERERSSRSAFIREAMRHYVWEKRKEHLRARMEAGYQEMARINLVMAVEAQPAEE
EADLTILRMVSGV

Nucleotide

• Length: 282 bp

>AT212359 NZ_CP107004:c1122209-1121838 [Escherichia coli]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA

Structures

Toxin

• AlphaFold DB: A0A132NF83