Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2160984..2161498 Replicon chromosome
Accession NZ_CP080770
Organism Staphylococcus aureus strain HL23187

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag HLV54_RS10715 Protein ID WP_000581792.1
Coordinates 2160984..2161193 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A0D1GBN9
Locus tag HLV54_RS10720 Protein ID WP_000138934.1
Coordinates 2161205..2161498 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLV54_RS10695 (HLV54_002139) 2156492..2157850 - 1359 WP_000611468.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
HLV54_RS10700 (HLV54_002140) 2157865..2158935 - 1071 WP_000159641.1 D-alanine--D-alanine ligase -
HLV54_RS10705 (HLV54_002141) 2159253..2160455 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
HLV54_RS10710 (HLV54_002142) 2160719..2160856 - 138 WP_000828354.1 Lmo0850 family protein -
HLV54_RS10715 (HLV54_002143) 2160984..2161193 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
HLV54_RS10720 (HLV54_002144) 2161205..2161498 - 294 WP_000138934.1 copper-sensing transcriptional repressor CsoR Antitoxin
HLV54_RS10725 (HLV54_002145) 2161664..2163148 + 1485 WP_000571549.1 cardiolipin synthase -
HLV54_RS10730 (HLV54_002146) 2163176..2163823 + 648 WP_001187610.1 HD domain-containing protein -
HLV54_RS10735 (HLV54_002147) 2164638..2165510 - 873 WP_000725802.1 membrane protein insertase YidC -
HLV54_RS10740 (HLV54_002148) 2165596..2166237 - 642 WP_000483147.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(3-95)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T212355 WP_000581792.1 NZ_CP080770:c2161193-2160984 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T212355 NZ_CP107004:714669-714965 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11185.77 Da        Isoelectric Point: 6.2243

>AT212355 WP_000138934.1 NZ_CP080770:c2161498-2161205 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT212355 NZ_CP107004:714967-715362 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB A0A0D1GBN9

References