Detailed information of TA system TA212354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2141244..2141773
• Replicon: chromosome
• Accession: NZ_CP080770
• Organism: Staphylococcus aureus strain HL23187

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HLV54_RS10625
• Protein ID: WP_000621175.1
• Coordinates: 2141244..2141606 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HLV54_RS10630
• Protein ID: WP_000948331.1
• Coordinates: 2141603..2141773 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLV54_RS10605 (HLV54_002121)	2138223..2138993	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
HLV54_RS10610 (HLV54_002122)	2138968..2139447	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
HLV54_RS10615 (HLV54_002123)	2139449..2139775	-	327	WP_001052491.1	anti-sigma factor antagonist	-
HLV54_RS10620 (HLV54_002124)	2139894..2140895	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
HLV54_RS10625 (HLV54_002125)	2141244..2141606	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HLV54_RS10630 (HLV54_002126)	2141603..2141773	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HLV54_RS10635 (HLV54_002127)	2141858..2143006	-	1149	WP_001280702.1	alanine racemase	-
HLV54_RS10640 (HLV54_002128)	2143072..2143431	-	360	WP_000581194.1	holo-ACP synthase	-
HLV54_RS10645 (HLV54_002129)	2143435..2143926	-	492	WP_001205915.1	PH domain-containing protein	-
HLV54_RS10650 (HLV54_002130)	2143913..2145496	-	1584	WP_001294642.1	PH domain-containing protein	-
HLV54_RS10655 (HLV54_002131)	2145489..2145968	-	480	WP_001287083.1	hypothetical protein	-
HLV54_RS10660 (HLV54_002132)	2146177..2146737	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T212354 WP_000621175.1 NZ_CP080770:c2141606-2141244 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T212354 NZ_CP107004:648758-649072 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT212354 WP_000948331.1 NZ_CP080770:c2141773-2141603 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT212354 NZ_CP107004:649069-649485 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1