Detailed information of TA system TA212346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF-ParD
• Location: 419842..420480
• Replicon: chromosome
• Accession: NZ_CP080764
• Organism: Aneurinibacillus thermoaerophilus strain CCM 8960

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: K3F53_RS02430
• Protein ID: WP_057899974.1
• Coordinates: 420130..420480 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K3F53_RS02425
• Protein ID: WP_057899975.1
• Coordinates: 419842..420126 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3F53_RS02400 (K3F53_02400)	415643..415807	-	165	WP_156424103.1	hypothetical protein	-
K3F53_RS02405 (K3F53_02405)	416448..416915	+	468	WP_091261626.1	hypothetical protein	-
K3F53_RS02410 (K3F53_02410)	417088..417600	-	513	WP_220559294.1	hypothetical protein	-
K3F53_RS02415 (K3F53_02415)	417954..418391	+	438	WP_091260563.1	hypothetical protein	-
K3F53_RS02420 (K3F53_02420)	418395..419579	+	1185	WP_057899976.1	alanine racemase	-
K3F53_RS02425 (K3F53_02425)	419842..420126	+	285	WP_057899975.1	ribbon-helix-helix protein, CopG family	Antitoxin
K3F53_RS02430 (K3F53_02430)	420130..420480	+	351	WP_057899974.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K3F53_RS02435 (K3F53_02435)	420572..422734	+	2163	WP_091260564.1	Tex family protein	-
K3F53_RS02440 (K3F53_02440)	422764..422883	-	120	WP_091260565.1	cortex morphogenetic protein CmpA	-
K3F53_RS02445 (K3F53_02445)	422974..423318	-	345	WP_091260566.1	hydrolase/acyltransferase	-
K3F53_RS02450 (K3F53_02450)	423418..423900	+	483	WP_057900438.1	SprT family protein	-
K3F53_RS02455 (K3F53_02455)	423881..424903	+	1023	WP_057899971.1	thiamine-phosphate kinase	-
K3F53_RS02460 (K3F53_02460)	424933..425412	+	480	WP_057899970.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12959.94 Da
• Isoelectric Point: 4.8668

>T212346 WP_057899974.1 NZ_CP080764:420130-420480 [Aneurinibacillus thermoaerophilus]
VIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYAFDRDSVILLEQI
RTIDKQRLTDKITHLDEEMMERVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T212346 NZ_CP107003:3743321-3743662 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10953.81 Da
• Isoelectric Point: 8.5616

>AT212346 WP_057899975.1 NZ_CP080764:419842-420126 [Aneurinibacillus thermoaerophilus]
MVLSKMDTKRIMISLPGHLLQEVDGLVEKEKSNRSEFIRQAMRLYLKERKKRHIREMMQRGYMEMANINLDIASEAFQAE
EEADLTLGRLVSGV

Nucleotide

• Length: 285 bp

>AT212346 NZ_CP107003:3742983-3743300 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA