Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 996927..998425 Replicon chromosome
Accession NZ_CP080761
Organism Diaphorobacter sp. MNS-0

Toxin (Protein)


Gene name rlegT Uniprot ID A0A7T1GP45
Locus tag K2L43_RS04770 Protein ID WP_011804354.1
Coordinates 996927..997829 (-) Length 301 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID A0A7T1GP65
Locus tag K2L43_RS04775 Protein ID WP_049756454.1
Coordinates 997826..998425 (-) Length 200 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K2L43_RS04745 (K2L43_04745) 992488..993174 + 687 WP_083766726.1 hypothetical protein -
K2L43_RS04750 (K2L43_04750) 993074..994308 + 1235 WP_085947515.1 IS3 family transposase -
K2L43_RS04755 (K2L43_04755) 994395..994763 + 369 WP_220638608.1 hypothetical protein -
K2L43_RS04765 (K2L43_04765) 996090..996914 + 825 WP_220638609.1 hypothetical protein -
K2L43_RS04770 (K2L43_04770) 996927..997829 - 903 WP_011804354.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
K2L43_RS04775 (K2L43_04775) 997826..998425 - 600 WP_049756454.1 AbiEi antitoxin N-terminal domain-containing protein Antitoxin
K2L43_RS04780 (K2L43_04780) 998498..999817 - 1320 WP_255580584.1 site-specific integrase -
K2L43_RS04785 (K2L43_04785) 999897..1000985 + 1089 WP_011803559.1 IS5-like element ISDisp1 family transposase -
K2L43_RS04790 (K2L43_04790) 1001521..1002024 - 504 WP_196995045.1 hypothetical protein -
K2L43_RS04795 (K2L43_04795) 1002091..1002483 - 393 WP_196995046.1 Rid family hydrolase -
K2L43_RS04800 (K2L43_04800) 1002595..1002843 + 249 Protein_962 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 993125..1004153 11028


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(35-257)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(7-52)


Sequences

Toxin        


Download         Length: 301 a.a.        Molecular weight: 32799.50 Da        Isoelectric Point: 5.8761

>T212343 WP_011804354.1 NZ_CP080761:c997829-996927 [Diaphorobacter sp. MNS-0]
MTGNLSASILARLLTLAKQRGDDYNLLLNRYGMERLLARVSTSPHADRFLLKGALLFALWYDTPHRPTRDADLLGFGPDD
EANLIATFRDIAAMDLGDGIVFDPESVKADAIREDNTYGGTRITLVARIGSARCALQIDVGFGDAVTPGPQTVAYPTLLG
DFPAPTLRVYPVYTVIAEKYQAMVMLGQANSRMKDFFDLAVIARRTELDGATLAAAIAATFARRQTALPTGRPLALTQQF
SEDAAKLRQWQAFLNKNRIEAASLGETVALLDDLLWPPTQVATASSQATATWRPEALRWV

Download         Length: 903 bp

>T212343 NZ_CP107003:2670509-2670616 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 200 a.a.        Molecular weight: 22482.01 Da        Isoelectric Point: 9.9492

>AT212343 WP_049756454.1 NZ_CP080761:c998425-997826 [Diaphorobacter sp. MNS-0]
MNQSDHILELARHRRVLRAADVREHGWSPQLLIRLHQAGKLQRLGRGLYGLPDAEVTEHQTLIEVCQRVPKAVLCLLSAL
QFHEIGTQLPHEVWIALPEATQTPALSYPTLRITRLRGTAYSNGIQTVTEHGAPIRVYSAAKTVADCFKFRNKIGLDVAL
EALKDAWRSRKVTIAELSHFAKINRVDRVMQPYLEAVTQ

Download         Length: 600 bp

>AT212343 NZ_CP107003:c2670462-2670396 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T1GP45

Antitoxin

Source ID Structure
AlphaFold DB A0A7T1GP65

References