Detailed information of TA system TA212342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1238245..1239162
• Replicon: chromosome
• Accession: NZ_CP080760
• Organism: Bacillus velezensis strain B4-7

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: K3A92_RS06430
• Protein ID: WP_007407256.1
• Coordinates: 1238416..1239162 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: K3A92_RS06425
• Protein ID: WP_003154807.1
• Coordinates: 1238245..1238415 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3A92_RS06390 (K3A92_06390)	1233478..1235100	+	1623	WP_032874601.1	pyocin knob domain-containing protein	-
K3A92_RS06395 (K3A92_06395)	1235113..1235484	+	372	WP_032874603.1	XkdW family protein	-
K3A92_RS06400 (K3A92_06400)	1235489..1235686	+	198	WP_032874605.1	XkdX family protein	-
K3A92_RS06405 (K3A92_06405)	1235743..1236504	+	762	WP_032874607.1	hypothetical protein	-
K3A92_RS06410 (K3A92_06410)	1236556..1236819	+	264	WP_003154815.1	hemolysin XhlA family protein	-
K3A92_RS06415 (K3A92_06415)	1236833..1237096	+	264	WP_003154813.1	phage holin	-
K3A92_RS06420 (K3A92_06420)	1237110..1237988	+	879	WP_032874609.1	N-acetylmuramoyl-L-alanine amidase	-
K3A92_RS19430	1238023..1238148	-	126	WP_003154809.1	hypothetical protein	-
K3A92_RS06425 (K3A92_06425)	1238245..1238415	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
K3A92_RS06430 (K3A92_06430)	1238416..1239162	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
K3A92_RS06435 (K3A92_06435)	1239267..1240265	-	999	WP_032874611.1	inorganic phosphate transporter	-
K3A92_RS06440 (K3A92_06440)	1240278..1240895	-	618	WP_032874614.1	DUF47 domain-containing protein	-
K3A92_RS06445 (K3A92_06445)	1241181..1242497	-	1317	WP_032874616.1	amino acid permease	-
K3A92_RS06450 (K3A92_06450)	1242819..1243769	+	951	WP_032874618.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T212342 WP_007407256.1 NZ_CP080760:c1239162-1238416 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T212342 NZ_CP107003:c2382413-2382237 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT212342 WP_003154807.1 NZ_CP080760:c1238415-1238245 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT212342 NZ_CP107003:c2382191-2381775 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14